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Two-photon fluorescent probes for detecting the viscosity of lipid droplets and its application in living cells.

ABSTRACT: Lipid droplets (LDs) are storage organelles at the centre of lipid and energy homeostasis, which act as vital hubs of cellular metabolism and the key to maintaining lipid and energy homeostasis. We synthesized a new two-photon fluorescent probe (CIV) that could detect the viscosity of lipid droplets. The probe is constructed via the typical ICT system of D-π-A using carbazole as the donor and imidazole as the acceptor. With the increase in viscosity from PBS to 99% glycerol, the fluorescence intensity of CIV increased by 13-fold, showing sensitivity and specificity towards viscosity. In addition, CIV showed low toxicity and excellent biocompatibility in cytotoxicity tests, and was successfully used for living cell LD imaging. Taken together, the results widen the way for the development of novel fluorescent probe-based the visualization LDs and detection in solutions, physiology and pathology.

SUBMITTER: Chen H

PROVIDER: S-EPMC8695079 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Two-photon fluorescent probes for detecting the viscosity of lipid droplets and its application in living cells.

Chen Huiying H Zhao Jianzhi J Lin Junzhi J Dong Baoli B Li Hui H Geng Bing B Yan Mei M

RSC advances 20210222 14

Lipid droplets (LDs) are storage organelles at the centre of lipid and energy homeostasis, which act as vital hubs of cellular metabolism and the key to maintaining lipid and energy homeostasis. We synthesized a new two-photon fluorescent probe (CIV) that could detect the viscosity of lipid droplets. The probe is constructed <i>via</i> the typical ICT system of D-π-A using carbazole as the donor and imidazole as the acceptor. With the increase in viscosity from PBS to 99% glycerol, the fluoresce ...[more]

PMID: 35423320

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