Metabolomics Diagnosis of COVID-19 from Exhaled Breath Condensate.
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ABSTRACT:
SUBMITTER: Barberis E
PROVIDER: S-EPMC8708149 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Metabolomics Diagnosis of COVID-19 from Exhaled Breath Condensate.
Barberis Elettra E Amede Elia E Khoso Shahzaib S Castello Luigi L Sainaghi Pier Paolo PP Bellan Mattia M Balbo Piero Emilio PE Patti Giuseppe G Brustia Diego D Giordano Mara M Rolla Roberta R Chiocchetti Annalisa A Romani Giorgia G Manfredi Marcello M Vaschetto Rosanna R
Metabolites 20211206 12
Infection from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can lead to severe respiratory tract damage and acute lung injury. Therefore, it is crucial to study breath-associated biofluids not only to investigate the breath's biochemical changes caused by SARS-CoV-2 infection, but also to discover potential biomarkers for the development of new diagnostic tools. In the present study, we performed an untargeted metabolomics approach using a bidimensional gas chromatography mass sp ...[more]
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Project description:Chronic obstructive pulmonary disease (COPD) is characterized by different phenotypes and clinical presentations. Therefore, a single strategy of pulmonary rehabilitation (PR) does not always yield the expected clinical outcomes as some individuals respond excellently, others discreetly, or do not respond at all. Fifty consecutive COPD patients were enrolled. Of them, 35 starting a 5-week PR program were sampled at admission (T0), after 2 (T2W) and 5 (T5W) weeks, while 15 controls not yet on PR were tested at T0 and T5W. Nuclear magnetic resonance (NMR) profiling of exhaled breath condensate (EBC) and multivariate statistical analysis were applied to investigate the relationship between biomarkers and clinical parameters. The model including the three classes correctly located T2W between T0 and T5W, but 38.71% of samples partially overlapped with T0 and 32.26% with T5W, suggesting that for some patients PR is already beneficial at T2W (32.26% overlapping with T5W), while for others (38.71% overlapping with T0) more time is required. Rehabilitated patients presented several altered biomarkers. In particular, methanol from T0 to T5W decreased in parallel with dyspnea and fatigue, while the walk distance increased. Methanol could be ascribed to lung inflammation. We demonstrated that the metabolic COPD phenotype clearly evolves during PR, with a strict relationship between clinical and molecular parameters. Methanol, correlating with clinical parameters, represents a useful biomarker for monitoring personalized outcomes and establishing more targeted protocols.
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2019-08-16 | PXD006436 | Pride
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