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Optical property recovery with spatially-resolved diffuse reflectance at short source-detector separations using a compact fiber-optic probe.

ABSTRACT: We describe a compact fiber-optic probe (2 mm outside diameter) that utilizes spatially-resolved diffuse reflectance for tissue optical property recovery. Validation was performed in phantoms containing Intralipid 20% as scatterer, and methylene blue (MB), MnTPPS, and/or India ink as absorbers. Over a range of conditions, the reduced scattering coefficient was recovered with a root mean square error (RMSE) of 0.86-2.7 cm^-1 (average error = 3.8%). MB concentration was recovered with RMSE = 0.26-0.52 µM (average error = 15.0%), which did not vary with inclusion of MnTPPS (p=0.65). This system will be utilized to determine optical properties in human abscesses, in order to generate treatment plans for photodynamic therapy.

SUBMITTER: Bridger KG

PROVIDER: S-EPMC8713658 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Optical property recovery with spatially-resolved diffuse reflectance at short source-detector separations using a compact fiber-optic probe.

Bridger Karina G KG Roccabruna Jacob R JR Baran Timothy M TM

Biomedical optics express 20211109 12

We describe a compact fiber-optic probe (2 mm outside diameter) that utilizes spatially-resolved diffuse reflectance for tissue optical property recovery. Validation was performed in phantoms containing Intralipid 20% as scatterer, and methylene blue (MB), MnTPPS, and/or India ink as absorbers. Over a range of conditions, the reduced scattering coefficient was recovered with a root mean square error (RMSE) of 0.86-2.7 cm<sup>-1</sup> (average error = 3.8%). MB concentration was recovered with RM ...[more]

PMID: 35003841

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Project description:SignificanceFiber-optic extended-wavelength diffuse reflectance spectroscopy (EWDRS) using both visible/near-infrared and shortwave-infrared detectors enables improved detection of spectral absorbances arising from lipids, water, and collagen and has demonstrated promise in a variety of applications, including detection of nerves and neurovascular bundles (NVB). Development of future applications of EWDRS for nerve detection could benefit from the use of model-based analyses including Monte Carlo (MC) simulations and evaluation of agreement between model systems and empirical measurements.AimThe aim of this work is to characterize agreement between EWDRS measurements and simulations and inform future applications of model-based studies of nerve-detecting applications.ApproachA model-based platform consisting of an ex vivo microsurgical nerve dissection model, unique two-layer optical phantoms, and MC model simulations of fiber-optic EWDRS spectroscopic measurements were used to characterize EWDRS and compare agreement across models. In addition, MC simulations of an EWDRS measurement scenario are performed to provide a representative example of future analyses.ResultsEWDRS studies performed in the common chicken thigh femoral nerve microsurgical dissection model indicate similar spectral features for classification of NVB versus adjacent tissues as reported in porcine models and human subjects. A comparison of measurements from unique EWDRS issue mimicking optical phantoms and MC simulations indicates high agreement between the two in homogeneous and two-layer optical phantoms, as well as in dissected tissues. Finally, MC simulations of measurement over a simulated NVB indicate the potential of future applications for measurement of nerve plexus.ConclusionsCharacterization of agreement between fiber-optic EWDRS measurements and MC simulations demonstrates strong agreement across a variety of tissues and optical phantoms, offering promise for further use to guide the continued development of EWDRS for translational applications.

Dataset Information

Optical property recovery with spatially-resolved diffuse reflectance at short source-detector separations using a compact fiber-optic probe.

Publications

Optical property recovery with spatially-resolved diffuse reflectance at short source-detector separations using a compact fiber-optic probe.

Similar Datasets

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