Unknown

Dataset Information

0

Quantifying phosphorylation dynamics in primary neuronal cultures using LC-MS/MS.


ABSTRACT: Cellular processes require tight and coordinated control of protein abundance, localization, and activity. One of the core mechanisms to achieve specific regulation of proteins is protein phosphorylation. Here we present a workflow to monitor protein abundance and phosphorylation in primary cultured neurons using liquid chromatography-coupled mass spectrometry. Our protocol provides a detailed guide on all steps for detection and label-free-quantification of phosphorylated and unmodified proteins of primary cortical neurons, including primary cell culture, phosphoproteomic sample preparation and data-processing, and evaluation. For complete details on the use and execution of this protocol, please refer to Desch et al. (2021).

SUBMITTER: Desch K 

PROVIDER: S-EPMC8715330 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2816933 | biostudies-literature
| S-EPMC8541004 | biostudies-literature
| S-EPMC6653605 | biostudies-literature
| S-EPMC7382969 | biostudies-literature
| S-EPMC3489469 | biostudies-literature
| S-EPMC3351829 | biostudies-literature
| S-EPMC7161314 | biostudies-literature
| S-EPMC10440349 | biostudies-literature
| S-EPMC8718357 | biostudies-literature
| S-EPMC9563341 | biostudies-literature