Ontology highlight
ABSTRACT:
SUBMITTER: Lee H
PROVIDER: S-EPMC87161 | biostudies-literature | 2000 Aug
REPOSITORIES: biostudies-literature
Lee H H Park H J HJ Cho S N SN Bai G H GH Kim S J SJ
Journal of clinical microbiology 20000801 8
PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species level. A total of 50 mycobacterial reference strains and 3 related bacterial strains were used to amplify the 360-bp region of rpoB, and the amplified DNAs were subsequently digested with restriction enzymes such as MspI and HaeIII. The results from this study clearly show that most of the mycobacterial species were ...[more]