Unknown

Dataset Information

0

Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene.

ABSTRACT: A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the restriction fragment profile was calculated using a computer-aided comparison program. An algorithm describing these 54 species (including 22 species not previously described) is proposed. We found that this assay based on 10% PAGE provided a more precise estimate than that based on agarose gel electrophoresis of the real size of restriction fragments as deduced from the sequence analysis and allowed identification of mycobacteria whose PCR-restriction fragment length polymorphism analysis patterns were unequivocally identified by fragments shorter than 60 bp.

SUBMITTER: Brunello F 

PROVIDER: S-EPMC88242 | biostudies-literature | 2001 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene.

Brunello F F   Ligozzi M M   Cristelli E E   Bonora S S   Tortoli E E   Fontana R R  

Journal of clinical microbiology 20010801 8

A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the restriction fragment profile was calculated using a computer-aided comparison program. An algorithm desc  ...[more]

Similar Datasets

Unknown Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene.
| S-EPMC87161 | biostudies-literature
Unknown Identification of Pneumococcal Serotypes by PCR-Restriction Fragment Length Polymorphism.
| S-EPMC6963424 | biostudies-literature
Unknown Species-specific identification of Campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene.
| S-EPMC2293133 | biostudies-literature
Unknown Development of a species-specific PCR-restriction fragment length polymorphism analysis procedure for identification of Madurella mycetomatis.
| S-EPMC85521 | biostudies-literature
Unknown Detection of cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism.
| S-EPMC1081268 | biostudies-literature
Unknown Autoscreening of restriction endonucleases for PCR-restriction fragment length polymorphism identification of fungal species, with Pleurotus spp. as an example.
| S-EPMC2168144 | biostudies-literature
Unknown Identification of infectious agents in onychomycoses by PCR-terminal restriction fragment length polymorphism.
| S-EPMC3295142 | biostudies-literature
Unknown Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.
| S-EPMC88409 | biostudies-literature
Unknown Genetic identification of the main opportunistic Mucorales by PCR-restriction fragment length polymorphism.
| S-EPMC1393117 | biostudies-literature
Unknown Phylogenetic analysis and PCR-restriction fragment length polymorphism identification of Campylobacter species based on partial groEL gene sequences.
| S-EPMC535295 | biostudies-literature