Ontology highlight
ABSTRACT:
SUBMITTER: Lindenburg LH
PROVIDER: S-EPMC8727024 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Lindenburg Laurens H LH Pantelejevs Teodors T Gielen Fabrice F Zuazua-Villar Pedro P Butz Maren M Rees Eric E Kaminski Clemens F CF Downs Jessica A JA Hyvönen Marko M Hollfelder Florian F
Proceedings of the National Academy of Sciences of the United States of America 20211101 46
Exchanges of protein sequence modules support leaps in function unavailable through point mutations during evolution. Here we study the role of the two RAD51-interacting modules within the eight binding BRC repeats of BRCA2. We created 64 chimeric repeats by shuffling these modules and measured their binding to RAD51. We found that certain shuffled module combinations were stronger binders than any of the module combinations in the natural repeats. Surprisingly, the contribution from the two mod ...[more]