ATP is an essential autocrine factor for pancreatic β-cell signaling and insulin secretion.

ABSTRACT: ATP has been previously identified as an autocrine signaling factor that is co-released with insulin to modulate and propagate β-cell activity within islets of Langerhans. Here, we show that β-cell activity and insulin secretion essentially rely on the presence of extracellular ATP. For this, we monitored changes of the intracellular Ca²⁺ concentration ([Ca²⁺ ]_i oscillations) as an immediate read-out for insulin secretion in live cell experiments. Extensive washing of cells or depletion of extracellular ATP levels by recombinant apyrase reduced [Ca²⁺ ]_i oscillations and insulin secretion in pancreatic cell lines and primary β-cells. Following ATP depletion, [Ca²⁺ ]_i oscillations were stimulated by the replenishmen

SUBMITTER: Hauke S 

PROVIDER: S-EPMC8743876 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature