Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry.

ABSTRACT: Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS) for proteomic analysis down to the single-cell level. The proteomics chips enable multiplexed and automated cell isolation/counting/imaging and sample processing in a single device. Combining chip-based sample handling with DIA-MS using project-specific mass spectral libraries, we profile on average ~1,500 protein groups across 20 single mammalian cells. Applying the chip-DIA workflow to profile the proteomes of adherent and non-adherent malignant cells, we cover a dynamic range of

SUBMITTER: Gebreyesus ST 

PROVIDER: S-EPMC8748772 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature