Dataset Information

Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing.

ABSTRACT:

Background

Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD).

Methods

Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD.

Results

Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p.

Conclusion

We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.

SUBMITTER: Chang SN

PROVIDER: S-EPMC8750494 | biostudies-literature |

REPOSITORIES: biostudies-literature

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