Dataset Information

MiR-423-5p prevents MALAT1-mediated proliferation and metastasis in prostate cancer.

ABSTRACT:

Background

The long non-coding RNA (lncRNA), MALAT1, plays a key role in the development of different cancers, and its expression is associated with worse prognosis in patients. However, its mechanism of action and its regulation are not well known in prostate cancer (PCa). A general mechanism of action of lncRNAs is their interaction with other epigenetic regulators including microRNAs (miRNAs).

Methods

Using lentiviral stable miRNA transfection together with cell biology functional assays and gene expression/target analysis, we investigated the interaction between MALAT1 and miR-423-5p, defined as a target with in silico prediction analysis, in PCa.

Results

Through bioinformatic analysis of data available from TCGA, we have found that MALAT1 expression correlates with high Gleason grade, metastasis occurrence, and reduced survival in PCa patients. These findings were validated on a TMA of PCa showing a significant correlation between MALAT1 expression with both stage and grading. We report that, in PCa cells, MALAT1 expression and activity is regulated by miR-423-5p that binds MALAT1, downregulates its expression and inhibits its activity in promoting proliferation, migration, and invasion. Using NanoString analysis, we unraveled downstream cell pathways that were affected by miR-423-5p expression and MALAT1 downregulation and identified several alterations in genes that are involved in metastatic response and angiogenic pathways. In addition, we showed that the overexpression of miR-423-5p increases survival and decreases metastases formation in a xenograft mouse model.

Conclusions

We provide evidence on the role of MALAT1 in PCa tumorigenesis and progression. Also, we identify a direct interaction between miR-423-5p and MALAT1, which results in the suppression of MALAT1 action in PCa.

SUBMITTER: Ferri C

PROVIDER: S-EPMC8751098 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Publications

MiR-423-5p prevents MALAT1-mediated proliferation and metastasis in prostate cancer.

Ferri Carmela C Di Biase Anna A Bocchetti Marco M Zappavigna Silvia S Wagner Sarah S Le Vu Pauline P Luce Amalia A Cossu Alessia Maria AM Vadakekolathu Jayakumar J Miles Amanda A Boocock David J DJ Robinson Alex A Schwerdtfeger Melanie M Tirino Virginia V Papaccio Federica F Caraglia Michele M Regad Tarik T Desiderio Vincenzo V

Journal of experimental & clinical cancer research : CR 20220111 1

<h4>Background</h4>The long non-coding RNA (lncRNA), MALAT1, plays a key role in the development of different cancers, and its expression is associated with worse prognosis in patients. However, its mechanism of action and its regulation are not well known in prostate cancer (PCa). A general mechanism of action of lncRNAs is their interaction with other epigenetic regulators including microRNAs (miRNAs).<h4>Methods</h4>Using lentiviral stable miRNA transfection together with cell biology functio ...[more]

PMID: 35016717

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Dataset Information

MiR-423-5p prevents MALAT1-mediated proliferation and metastasis in prostate cancer.

Background

Methods

Results

Conclusions

Publications

MiR-423-5p prevents MALAT1-mediated proliferation and metastasis in prostate cancer.

Similar Datasets

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