Wearable AIEgen-Based Lateral Flow Test Strip for Rapid Detection of SARS-CoV-2 RBD Protein and N Protein.

ABSTRACT: Accurate and rapid detection of SARS-CoV-2 is significant for early tracing, isolating and treating infected patients, which will efficiently prevent large-scale transmission of COVID-19. Herein, two kinds of test strips for RBD and N antigens of SARS-CoV-2 are established with high sensitivity and specificity, in which AIE luminogens are utilized as reporters. Because of the high brightness and resistance of quenching properties in aqueous solution, the limit of detection can be as low as 6.9 ng/mL for RBD protein and 7.2 ng/mL for N protein. As an antigen collector, the N95 mask equipped with the test strip with excellent enrichment effect would efficiently simplify the sampling procedures. Compared with the test strip based on Au nanoparticles or FITC, the AIEgen-based test strip shows high anti-interference capacity in complex biosamples. Therefore, the AIEgen-based test strip assay could be built as a promising platform for emergency usage during the pandemic.

SUBMITTER: Zhang GQ 

PROVIDER: S-EPMC8761541 | biostudies-literature |

REPOSITORIES: biostudies-literature