Dataset Information

Development of 1,5-Diaryl-Pyrazole-3-Formate Analogs as Antifungal Pesticides and Their Application in Controlling Peanut Stem Rot Disease.

ABSTRACT: Stem rot disease caused by Sclerotium rolfsii is one of the destructive diseases in peanut and poses a big risk to peanut production. Current fungicides in the market have not provided satisfactory control efficacy and thus called for novel fungicides with different structures as an alternative treatment strategy. Our previously developed phenylpyrazole compound 3c demonstrated modest inhibitory effect against S. rolfsii. The following structure modification identified an unreported compound 6, which bears a 3-chloropyridinyl moiety as the most prominent derivative with an IC₅₀ of 12 μg/ml in potato dextrose agar (PDA) assay, higher than those of 0.8 and 1.8 μg/ml associated with thifluzamide and tebuconazole, respectively. However, compound 6 showed similar controlling effects to those of thifluzamide and tebuconazole in field study. This study underscores the potential of 1,5-diaryl-pyrazole-3-formate as an antifungal candidate for stem rot disease management.

SUBMITTER: Ma Y

PROVIDER: S-EPMC8763808 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Development of 1,5-Diaryl-Pyrazole-3-Formate Analogs as Antifungal Pesticides and Their Application in Controlling Peanut Stem Rot Disease.

Ma Yihui Y Yang Jun J Yang Dangwei D Qin Guangyu G Zu Junhuai J

Frontiers in microbiology 20220104

Stem rot disease caused by Sclerotium rolfsii is one of the destructive diseases in peanut and poses a big risk to peanut production. Current fungicides in the market have not provided satisfactory control efficacy and thus called for novel fungicides with different structures as an alternative treatment strategy. Our previously developed phenylpyrazole compound 3c demonstrated modest inhibitory effect against S. rolfsii. The following structure modification identified an un ...[more]

PMID: 35058889

Similar Datasets

Project description:Peanut stem rot, caused by Sclerotium rolfsii, considerably affects crop productivity. Application of chemical fungicides harms the environment and induces drug resistance. Biological agents are valid and eco-friendly alternatives to chemical fungicides. Bacillus spp. are important biocontrol agents that are now widely used against several plant diseases. This study aimed to evaluate the efficacy and mechanism of a potential biocontrol agent Bacillus sp. for controlling peanut stem rot caused by S. rolfsii. Here, we isolated a strain of Bacillus from pig biogas slurry that considerably inhibits the radial growth of S. rolfsii. The strain CB13 was identified as Bacillus velezensis on the basis of morphological, physiological, biochemical characteristics and phylogenetic trees based on the 16S rDNA and gyrA, gyrB, and rpoB gene sequences. The biocontrol efficacy of CB13 was evaluated on the basis of colonization ability, induction of defense enzyme activity, and soil microbial diversity. The control efficiencies of B. velezensis CB13-impregnated seeds in four pot experiments were 65.44, 73.33, 85.13, and 94.92%. Root colonization was confirmed through green fluorescent protein (GFP)-tagging experiments. The CB13-GFP strain was detected in peanut root and rhizosphere soil, at 104 and 108 CFU/g, respectively, after 50 days. Furthermore, B. velezensis CB13 enhanced the defense response against S. rolfsii infection by inducing defense enzyme activity. MiSeq sequencing revealed a shift in the rhizosphere bacterial and fungal communities in peanuts treated with B. velezensis CB13. Specifically, the treatment enhanced disease resistance by increasing the diversity of soil bacterial communities in peanut roots, increasing the abundance of beneficial communities, and promoting soil fertility. Additionally, real-time quantitative polymerase chain reaction results showed that B. velezensis CB13 stably colonized or increased the content of Bacillus spp. in the soil and effectively inhibited S. rolfsii proliferation in soil. These findings indicate that B. velezensis CB13 is a promising agent for the biocontrol of peanut stem rot.

Dataset Information

Development of 1,5-Diaryl-Pyrazole-3-Formate Analogs as Antifungal Pesticides and Their Application in Controlling Peanut Stem Rot Disease.

Publications

Development of 1,5-Diaryl-Pyrazole-3-Formate Analogs as Antifungal Pesticides and Their Application in Controlling Peanut Stem Rot Disease.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets