Browse
Submit Data
Databases
API
Help

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Disposable Stainless-Steel Wire-Based Electrochemical Microsensor for In Vivo Continuous Monitoring of Hydrogen Peroxide in Vein of Tomato Leaf.

ABSTRACT: As one of the pivotal signal molecules, hydrogen peroxide (H₂O₂) has been demonstrated to play important roles in many physiological processes of plants. Continuous monitoring of H₂O₂ in vivo could help understand its regulation mechanism more clearly. In this study, a disposable electrochemical microsensor for H₂O₂ was developed. This microsensor consists of three parts: low-cost stainless-steel wire with a diameter of 0.1 mm modified by gold nanoparticles (disposable working electrode), an untreated platinum wire with a diameter of 0.1 mm (counter electrode), and an Ag/AgCl wire with a diameter of 0.1 mm (reference electrode), respectively. The microsensor could detect H₂O₂ in levels from 10 to 1000 µM and exhibited excellent selectivity. On this basis, the dynamic change in H₂O₂ in the vein of tomato leaf under high salinity was continuously monitored in vivo. The results showed that the production of H₂O₂ could be induced by high salinity within two hours. This study suggests that the disposable electrochemical microsensor not only suits continuously detecting H₂O₂ in microscopic plant tissue in vivo but also reduces the damage to plants. Overall, our strategy will help to pave the foundation for further investigation of the generation, transportation, and elimination mechanism of H₂O₂ in plants.

SUBMITTER: Huo D

PROVIDER: S-EPMC8773776 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Optimizing Silanization to Functionalize Stainless Steel Wire: Towards Breast Cancer Stem Cell Isolation

Project description:Chemically modified metal surfaces have been used to recognize and capture specific cell types and biomolecules. In this work, stainless steel wires were functionalized with aptamers against breast cancer stem cell markers. Stainless steel wires were first electropolished and silanized via electrodeposition. Aptamers were then attached to the silanized surface through a cross-linker. The functionalized wires were able to capture the target cells in an in vitro test. During surface modification steps, wires were analyzed by atomic force microscopy, cyclic voltammetry, scanning electron and fluorescence microscopy to determine their surface composition and morphology. Optimized conditions of silanization (applied potential, solution pH, heat treatment temperature) for obtaining an aptamer-functionalized wire were determined in this work together with the use of several surface characterization techniques suitable for small-sized and circular wires. These modified wires have potential applications for the in vivo capture of target cells in blood flow, since their small size allows their insertion as standard guidewires in biomedical devices.

| S-EPMC7504676 | biostudies-literature

Inhibition of Bacterial Adhesion on Nanotextured Stainless Steel 316L by Electrochemical Etching.

Project description:Bacterial adhesion to stainless steel 316L (SS316L), which is an alloy typically used in many medical devices and food processing equipment, can cause serious infections along with substantial healthcare costs. This work demonstrates that nanotextured SS316L surfaces produced by electrochemical etching effectively inhibit bacterial adhesion of both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, but exhibit cytocompatibility and no toxicity toward mammalian cells in vitro. Additionally, the electrochemical surface modification on SS316L results in formation of superior passive layer at the surface, improving corrosion resistance. The nanotextured SS316L offers significant potential for medical applications based on the surface structure-induced reduction of bacterial adhesion without use of antibiotic or chemical modifications while providing cytocompatibility and corrosion resistance in physiological conditions.

| S-EPMC5761049 | biostudies-literature

Effect of nano-grain carbide formation on electrochemical behavior of 316L stainless steel.

Project description:The effect of low oxygen-partial pressured carburizing on relaxation process for 316L stainless steel is reported. Phase, morphology, and amount of compound formation during initial stage of carburizing are investigated using X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS). The results show formation and development of surface multilayer with nano-grain-carbide (Cr7C3, Fe7C3, and/or Cr3C2) generation in the layer located below outermost protective layer. The relaxation process has been investigated using electrochemical impedance spectroscopy (EIS). Formation of nano-grain carbide(s) during carburizing causes deterioration effect on the electrochemical behavior of steel. However, the steel with large amount of carbide generation (carburized for 30 min) tends to have higher corrosion resistance (indicated by higher values of Rcl and Rct) than the smaller ones (10 and 20 min) due to the effect of phase, grain size, morphology, and amount of compound formation.

| S-EPMC8206347 | biostudies-literature

Mouse Lung Response to Stainless Steel and Mild Steel Welding Fume

Project description:A/J mice are genetically predisposed to spontaneous and/or chemically-induced lung tumors while C57BL/6J (B6) mice are resistant. This genetic disparity provides a unique scenario to identify molecular mechanisms associated with the lung response to welding fume at the transcriptome level.

2010-04-30 | GSE20174 | GEO

Coupling mechanism between wear and oxidation processes of 304 stainless steel in hydrogen peroxide environments.

Project description:Stainless steel is widely used in strongly oxidizing hydrogen peroxide (H2O2) environments. It is crucial to study its wear behaviour and failure mode. The tribological properties and oxidation of 304 stainless steel were investigated using a MMW-1 tribo-tester with a three-electrode setup in H2O2 solutions with different concentrations. Corrosion current densities (CCDs), coefficients of frictions (COFs), wear mass losses, wear surface topographies, and metal oxide films were analysed and compared. The results show that the wear process and oxidation process interacted significantly with each other. Increasing the concentration of H2O2 or the oxidation time was useful to form a layer of integrated, homogeneous, compact and thick metal oxide film. The dense metal oxide films with higher mechanical strengths improved the wear process and also reduced the oxidation reaction. The wear process removed the metal oxide films to increase the oxidation reaction. Theoretical data is provided for the rational design and application of friction pairs in oxidation corrosion conditions.

| S-EPMC5443796 | biostudies-literature

Electrochemical Descaling of Metal Oxides from Stainless Steel Using an Ionic Liquid-Acid Solution.

Project description:Oxide scales often formed on the surface of stainless steel, and it is of high interest to descale the surface oxide effectively and environment-friendly during steel smelting and engineering processing. It is generally done by treating the oxide layer under strong and harsh mixed acid (HNO3 + HF) conditions or in a strong molten salt (NaOH + NaNO3) environment at high temperatures, while the generation of very harmful and environmentally hazardous gases, such as NO x , is inevitable. A novel, simple, fast, and environment-friendly electrochemical method at ambient temperature is proposed in this research to remove the oxide scale from the stainless steel surface using an ionic liquid with a small amount of HCl. It was found that the optimized electrochemical anodization treatment in an ionic liquid environment could significantly improve the descaling efficiency at least 50 times faster than the simple passive and slow dissolution in a mixture of an ionic liquid and a concentrated acid.

| S-EPMC7331208 | biostudies-literature

Electrochemical and passivation behavior investigation of ferritic stainless steel in simulated concrete pore media.

Project description:The applications of stainless steel are one of the most reliable solutions in concrete structures to reduce chloride-induced corrosion problems and increase the structures service life, however, due to high prices of nickel, especially in many civil engineering projects, the austenitic stainless steel is replaced by the ferritic stainless steels. Compared with austenite stainless steel, the ferritic stainless steel is known to be extremely resistant of stress corrosion cracking and other properties. The good corrosion resistance of the stainless steel is due to the formation of passive film. While, there is little literature about the electrochemical and passive behavior of ferritic stainless steel in the concrete environments. So, here, we present the several corrosion testing methods, such as the potentiodynamic measurements, EIS and Mott-Schottky approach, and the surface analysis methods like XPS and AES to display the passivation behavior of 430 ferritic stainless steel in alkaline solution with the presence of chloride ions. These research results illustrated a simple and facile approach for studying the electrochemical and passivation behavior of stainless steel in the concrete pore environments.

| S-EPMC4588451 | biostudies-literature

Evaluation of the thermal aging of ?-ferrite in austenitic stainless steel welds by electrochemical analysis.

Project description:Cr-segregation by spinodal decomposition and G-phase precipitation were observed in ?-ferrite of austenitic stainless steel welds thermally aged at 400 °C for up to 20,000?h. A reversion heat treatment (R-HT) at 550 °C for 1?h dissolved the Cr-segregation in the aged welds while some intermetallic precipitates were present. The double-loop electrochemical potentiokinetic reactivation (DL-EPR) analysis showed no significant differences among them. However, after selective etching of the austenite phase, the DL-EPR values of ?-ferrite phase steadily increased with aging time due to the growth of Cr-depleted regions by spinodal decomposition. The electrochemical behavior of ?-ferrite after R-HT condition was similar to that of unaged welds, indicating the intermetallic precipitates did not affect the corrosion resistance in this case. Overall, DL-EPR analysis of ?-ferrite phase provided better correlation with spinodal decomposition.

| S-EPMC6179992 | biostudies-literature

Electrochemical Bacterial Enrichment from Natural Seawater and Its Implications in Biocorrosion of Stainless-Steel Electrodes.

Project description:Microbial electrochemical technologies have revealed the opportunity of electrochemical enrichment for specific bacterial groups that are able to catalyze reactions of interest. However, there are unsolved challenges towards their application under aggressive environmental conditions, such as in the sea. This study demonstrates the impact of surface electrochemical potential on community composition and its corrosivity. Electrochemical bacterial enrichment was successfully carried out in natural seawater without nutrient amendments. Experiments were carried out for ten days of exposure in a closed-flow system over 316L stainless steel electrodes under three different poised potentials (-150 mV, +100 mV, and +310 mV vs. Ag/AgCl). Weight loss and atomic force microscopy showed a significant difference in corrosion when +310 mV (vs. Ag/AgCl) was applied in comparison to that produced under the other tested potentials (and an unpoised control). Bacterial community analysis conducted using 16S rRNA gene profiles showed that poised potentials are more positive as +310 mV (vs. Ag/AgCl) resulted in strong enrichment for Rhodobacteraceae and Sulfitobacter. Hence, even though significant enrichment of the known electrochemically active bacteria from the Rhodobacteraceae family was accomplished, the resultant bacterial community could accelerate pitting corrosion in 316 L stainless steel, thereby compromising the durability of the electrodes and the microbial electrochemical technologies.

| S-EPMC7288148 | biostudies-literature

Stainless Steel Wire Mesh Supported Molecularly Imprinted Composite Membranes for Selective Separation of Ebracteolata Compound B from <i>Euphorbia fischeriana</i>.

Project description:Stainless steel wire mesh supported molecularly imprinted composite membranes for selective separation of Ebracteolata Compound B (ECB) were prepared based on surface polymerization using ECB separated from Euphorbia fischeriana as a template, acrylamide as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, azodiisobutyronitrile as an initiator, and stainless steel wire mesh as support. Structure and purity of ECB were characterized by nuclear magenetic resonance (¹H-NMR, 13C-NMR) and ultra high performance liquid chromatography (UHPLC). The molecularly imprinted composite membranes were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The membrane adsorbed on the ECB reached equilibrium about 30 min later, with a maximum adsorption amount of 3.39 μmol/cm². Adsorption behavior between ECB and the molecularly imprinted composite membranes followed pseudo-second-order kinetics equation and Freundlich isotherm model. The molecularly imprinted composite membranes that could selectively identify and transport ECB in similar structures have a permeation rate of 38.71% to ECB. The ECB content in the permeation solution derived from the extract of Euphorbia fischeriana through the imprinted membrane was 87%. Overall, the obtained results demonstrated that an efficient approach with the molecularly imprinted composite membranes for selective separation of ECB from Euphorbia fischeriana.

| S-EPMC6384690 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data