Unknown

Dataset Information

0

DEAD-Box RNA Helicase DDX47 Maintains Midgut Homeostasis in Locusta migratoria.


ABSTRACT: Tissue homeostasis is critical for maintaining organ shape, size, and function. The condition is regulated by the balance between the generation of new cells and the loss of senescent cells, and it involves many factors and mechanisms. The midgut, an important part of the intestinal tract, is responsible for digestion and nutrient absorption in insects. LmDDX47, the ortholog of DEAD-box helicase 47 from Locusta migratoria, is indispensable for sustaining a normal midgut in the nymphs. However, the underlying cellular and molecular mechanisms remain to be elucidated. In this study, LmDDX47 knockdown resulted in atrophy of the midgut and gastric cecum in both nymph and adult locusts. After LmDDX47 knockdown, the number of regenerative and columnar cells in the midgut was significantly reduced, and cell death was induced in columnar tissue. LmDDX47 was localized to the nucleolus; this was consistent with the reduction in 18S rRNA synthesis in the LmDDX47 knockdown group. In addition, the acetylation and crotonylation levels of midgut proteins were significantly increased. Therefore, LmDDX47 could be a key regulator of midgut homeostasis, regulating 18S rRNA synthesis as well as protein acetylation and crotonylation in the migratory locust.

SUBMITTER: Wang JX 

PROVIDER: S-EPMC8775783 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7392221 | biostudies-literature
| S-EPMC2216682 | biostudies-literature
| S-EPMC5733242 | biostudies-literature
| S-EPMC4135551 | biostudies-literature
| S-EPMC4605566 | biostudies-literature
| S-EPMC4211656 | biostudies-literature
| S-EPMC9399600 | biostudies-literature
| S-EPMC27181 | biostudies-literature
| S-EPMC2577925 | biostudies-literature
| S-EPMC3280988 | biostudies-literature