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Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance.

ABSTRACT: The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.

SUBMITTER: Yano TA

PROVIDER: S-EPMC8776812 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance.

Yano Taka-Aki TA Kajisa Taira T Ono Masayuki M Miyasaka Yoshiya Y Hasegawa Yuichi Y Saito Atsushi A Otsuka Kunihiro K Sakane Ayuko A Sasaki Takuya T Yasutomo Koji K Hamajima Rina R Kanai Yuta Y Kobayashi Takeshi T Matsuura Yoshiharu Y Itonaga Makoto M Yasui Takeshi T

Scientific reports 20220120 1

The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD o ...[more]

PMID: 35058513

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