Regulation of Clostridium tetani Neurotoxin Expression by Culture Conditions.

ABSTRACT:

Background

Ensuring consistency of tetanus neurotoxin (TeNT) production by Clostridium tetani could help to ensure consistent product quality in tetanus vaccine manufacturing, ultimately contributing to reduced animal testing. The aim of this study was to identify RNA signatures related to consistent TeNT production using standard and non-standard culture conditions.

Methods

We applied RNA sequencing (RNA-Seq) to study C. tetani gene expression in small-scale batches under several culture conditions.

Results

We identified 1381 time-dependent differentially expressed genes (DEGs) reflecting, among others, changes in growth rate and metabolism. Comparing non-standard versus standard culture conditions identified 82 condition-dependent DEGs, most of which w

SUBMITTER: Pennings JLA 

PROVIDER: S-EPMC8778922 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature