SGIP1 is involved in regulation of emotionality, mood, and nociception and modulates in vivo signalling of cannabinoid CB₁ receptors.

ABSTRACT:

Background and purpose

Src homology 3-domain growth factor receptor-bound 2-like endophilin interacting protein 1 (SGIP1) interacts with cannabinoid CB₁ receptors. SGIP1 is abundantly and principally expressed within the nervous system. SGIP1 and CB₁ receptors co-localize in axons and presynaptic boutons. SGIP1 interferes with the internalization of activated CB₁ receptors in transfected heterologous cells. Consequently, the transient association of CB₁ receptors with β-arrestin2 is enhanced and prolonged, and CB₁ receptor-mediated ERK1/2 signalling is decreased. Because of these actions, SGIP1 may modulate affect, anxiety, pain processing, and other physiological processes controlled by the endocannabinoid system (ECS).

Experimental approach

Using a battery of behavioural tests, we investigated the consequences of SGIP1 deletion in tasks regulated by the ECS in SGIP1 constitutive knockout (SGIP1^-/- ) mice.

Key results

In SGIP1^-/- mice, sensorimotor gating, exploratory levels, and working memory are unaltered. SGIP1^-/- mice have decreased anxiety-like behaviours. Fear extinction to tone is facilitated in SGIP1^-/- females. Several cannabinoid tetrad behaviours are altered in the absence of SGIP1. SGIP1^-/- males exhibit abnormal behaviours on Δ⁹ -tetrahydrocannabinol withdrawal. SGIP1 deletion also reduces acute nociception, and SGIP1^-/- mice are more sensitive to analgesics.

Conclusion and implications

SGIP1 was detected as a novel protein associated with CB₁ receptors, and profoundly modified CB₁ receptor signalling. Genetic deletion of SGIP1 particularly affected behavioural tests of mood-related assessment and the cannabinoid tetrad. SGIP1^-/- mice exhibit decreased nociception and augmented responses to CB₁ receptor agonists and morphine. These in vivo findings suggest that SGIP1 is a novel modulator of CB₁ receptor-mediated behaviour.

SUBMITTER: Dvorakova M 

PROVIDER: S-EPMC8795748 | biostudies-literature |

REPOSITORIES: biostudies-literature