Ontology highlight
ABSTRACT:
SUBMITTER: Reischl U
PROVIDER: S-EPMC88058 | biostudies-literature | 2001 May
REPOSITORIES: biostudies-literature
Reischl U U Lehn N N Sanden G N GN Loeffelholz M J MJ
Journal of clinical microbiology 20010501 5
Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be ...[more]