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Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.


ABSTRACT: Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.

SUBMITTER: Guthrie JL 

PROVIDER: S-EPMC2849596 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.

Guthrie J L JL   Robertson A V AV   Tang P P   Jamieson F F   Drews S J SJ  

Journal of clinical microbiology 20100224 4


Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms. ...[more]

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