Dataset Information

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803.

ABSTRACT: Phycocyanin (PC) is a soluble blue pigment-protein primarily harvested from the cyanobacterium Arthrospira platensis. PC is in high demand from several industries, but its narrow stability range limits potential applications. Here, a pilot scale (120 L total) batch production, extraction and purification process for thermostable PC (Te-PC) from a Synechocystis sp. PCC 6803 'Olive' strain expressing the PC operon cpcBACD from Thermosynechococcus elongatus BP-1 on a self-replicating vector is presented. Batch cultivation without antibiotics had no impact on growth or Te-PC production and optimisation of growth conditions resulted in Te-PC contents of 75.3 ± 1.7 mg g DW^-1. Wet biomass was harvested following chitosan-based flocculation with a 97 ± 2% efficiency, and Te-PC was extracted by high pressure homogenisation. Subsequent purification by heat-treatment and two-step ammonium sulfate precipitation removed chlorophyll and allophycocyanin contamination, resulting in Te-PC purities of 2.9 ± 0.7 and a mean Te-PC recovery of 84 ± 12%.

SUBMITTER: Puzorjov A

PROVIDER: S-EPMC8811538 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803.

Puzorjov Anton A Mert Unal Suleyman S Wear Martin A MA McCormick Alistair J AJ

Bioresource technology 20211201

Phycocyanin (PC) is a soluble blue pigment-protein primarily harvested from the cyanobacterium Arthrospira platensis. PC is in high demand from several industries, but its narrow stability range limits potential applications. Here, a pilot scale (120 L total) batch production, extraction and purification process for thermostable PC (Te-PC) from a Synechocystis sp. PCC 6803 'Olive' strain expressing the PC operon cpcBACD from Thermosynechococcus elongatus BP-1 on a self-replicating vector is pres ...[more]

PMID: 34863843

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Dataset Information

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803.

Publications

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803.

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