Unknown

Dataset Information

0

Structure of Tetrahelical DNA Homopolymers Supports Quadruplex World Hypothesis.


ABSTRACT: We previously reported a tetrahelical monomolecular architecture of DNA, tmDNA, which employs G-quartets and an all-parallel GGGTGGGTGGGTGGG (G3T) quadruplex as the repeating unit. Based on thermodynamic and kinetic studies, we proposed that covalently joined (G3T) n units formed an uninterrupted programmable homopolymer; however, structural evidence for the tmDNA architecture was lacking. Here, we used NMR spectroscopy of wild-type and single-inosine-substituted constructs to characterize both monomolecular (G3T)2 and bimolecular quadruplex-Mg-coupled versions of tmDNA. The NMR results support an architecture consisting of uninterrupted stacked G-tetrads in both the monomolecular constructs and bimolecular assemblies. Taken together, these data support the formation of a stable programmable homopolymeric tmDNA architecture, which may have been a precursor to the modern-day Watson-Crick DNA duplex.

SUBMITTER: Lomidze L 

PROVIDER: S-EPMC8829921 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure of Tetrahelical DNA Homopolymers Supports Quadruplex World Hypothesis.

Lomidze Levan L   Yang Mengkun M   Khutsishvili David D   Metreveli Nunu N   Musier-Forsyth Karin K   Kankia Besik B  

ACS omega 20220126 5


We previously reported a tetrahelical monomolecular architecture of DNA, tmDNA, which employs G-quartets and an all-parallel GGGTGGGTGGGTGGG (G3T) quadruplex as the repeating unit. Based on thermodynamic and kinetic studies, we proposed that covalently joined (G3T) <i><sub>n</sub></i> units formed an uninterrupted programmable homopolymer; however, structural evidence for the tmDNA architecture was lacking. Here, we used NMR spectroscopy of wild-type and single-inosine-substituted constructs to  ...[more]

Similar Datasets

| S-EPMC11411666 | biostudies-literature
| S-EPMC6118410 | biostudies-literature
| S-EPMC4900677 | biostudies-literature
| S-EPMC1636468 | biostudies-literature
| S-EPMC5442326 | biostudies-literature
| S-EPMC4352798 | biostudies-literature
| S-EPMC5540935 | biostudies-literature
| S-EPMC7455006 | biostudies-literature
| S-EPMC5499593 | biostudies-literature
| S-EPMC10949190 | biostudies-literature