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Quantitative interactome analysis with chemical cross-linking and mass spectrometry.

ABSTRACT: Structural plasticity and dynamic protein-protein interactions are critical determinants of protein function within living systems. Quantitative chemical cross-linking with mass spectrometry (qXL-MS) is an emerging technology able to provide information on changes in protein conformations and interactions. Importantly, qXL-MS is applicable to complex biological systems, including living cells and tissues, thereby providing insights into proteins within their native environments. Here, we present an overview of recent technological developments and applications involving qXL-MS, including design and synthesis of isotope-labeled cross-linkers, development of new liquid chromatography-MS methodologies, and computational developments enabling interpretation of the data.

SUBMITTER: Wippel HH

PROVIDER: S-EPMC8837725 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Quantitative interactome analysis with chemical cross-linking and mass spectrometry.

Wippel Helisa H HH Chavez Juan D JD Tang Xiaoting X Bruce James E JE

Current opinion in chemical biology 20210812

Structural plasticity and dynamic protein-protein interactions are critical determinants of protein function within living systems. Quantitative chemical cross-linking with mass spectrometry (qXL-MS) is an emerging technology able to provide information on changes in protein conformations and interactions. Importantly, qXL-MS is applicable to complex biological systems, including living cells and tissues, thereby providing insights into proteins within their native environments. Here, we present ...[more]

PMID: 34393043

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