Rapid PCR-single-strand conformation polymorphism method to differentiate and estimate relative abundance of Pneumocystis carinii special forms infecting rats.
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ABSTRACT:
SUBMITTER: Nahimana A
PROVIDER: S-EPMC88588 | biostudies-literature | 2001 Dec
REPOSITORIES: biostudies-literature
Publications
Rapid PCR-single-strand conformation polymorphism method to differentiate and estimate relative abundance of Pneumocystis carinii special forms infecting rats.
Nahimana A A Cushion M T MT Blanc D S DS Hauser P M PM
Journal of clinical microbiology 20011201 12
A rapid method that uses PCR-single-strand conformation polymorphism analysis of the intron of the nuclear 26S rRNA gene was shown to differentiate the two Pneumocystis carinii special forms that infect rats, P. carinii f. sp. carinii and P. carinii f. sp. ratti. The method also provides a means for estimation of the relative abundance of the two special forms in the case of a coinfected rat. The results suggest that the method described will help to further standardize the immunosuppressed rat ...[more]
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