Dataset Information

Photoacoustic imaging of 3D-printed vascular networks.

ABSTRACT: Thrombosis in the circulation system can lead to major myocardial infarction and cardiovascular deaths. Understanding thrombosis formation is necessary for developing safe and effective treatments. In this work, using digital light processing (DLP)-based 3D printing, we fabricated sophisticatedin vitromodels of blood vessels with internal microchannels that can be used for thrombosis studies. In this regard, photoacoustic microscopy (PAM) offers a unique advantage for label-free visualization of the 3D-printed vessel models, with large penetration depth and functional sensitivity. We compared the imaging performances of two PAM implementations: optical-resolution PAM and acoustic-resolution PAM, and investigated 3D-printed vessel structures with different patterns of microchannels. Our results show that PAM can provide clear microchannel structures at depths up to 3.6 mm. We further quantified the blood oxygenation in the 3D-printed vascular models, showing that thrombi had lower oxygenation than the normal blood. We expect that PAM can find broad applications in 3D printing and bioprinting forin vitrostudies of various vascular and other diseases.

SUBMITTER: Ma C

PROVIDER: S-EPMC8885332 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Publications

Photoacoustic imaging of 3D-printed vascular networks.

Ma Chenshuo C Li Wanlu W Li Daiwei D Chen Maomao M Wang Mian M Jiang Laiming L Mille Luis Santiago LS Garciamendez Carlos Ezio CE Zhao Zhibo Z Zhou Qifa Q Zhang Yu Shrike YS Yao Junjie J

Biofabrication 20220124 2

Thrombosis in the circulation system can lead to major myocardial infarction and cardiovascular deaths. Understanding thrombosis formation is necessary for developing safe and effective treatments. In this work, using digital light processing (DLP)-based 3D printing, we fabricated sophisticated<i>in vitro</i>models of blood vessels with internal microchannels that can be used for thrombosis studies. In this regard, photoacoustic microscopy (PAM) offers a unique advantage for label-free visualiza ...[more]

PMID: 35008080

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Project description:Photoacoustic (PA) imaging-a technique combining the ability of optical imaging to probe functional properties of the tissue and deep structural imaging ability of ultrasound-has gained significant popularity in the past two decades for its utility in several biomedical applications. More recently, light-emitting diodes (LED) are being explored as an alternative to bulky and expensive laser systems used in PA imaging for their portability and low-cost. Due to the large beam divergence of LEDs compared to traditional laser beams, it is imperative to quantify the angular dependence of LED-based illumination and optimize its performance for imaging superficial or deep-seated lesions. A custom-built modular 3-D printed hinge system and tissue-mimicking phantoms with various absorption and scattering properties were used in this study to quantify the angular dependence of LED-based illumination. We also experimentally calculated the source divergence of the pulsed-LED arrays to be 58° ± 8°. Our results from point sources (pencil lead phantom) in non-scattering medium obey the cotangential relationship between the angle of irradiation and maximum PA intensity obtained at various imaging depths, as expected. Strong dependence on the angle of illumination at superficial depths (-5°/mm at 10 mm) was observed that becomes weaker at intermediate depths (-2.5°/mm at 20 mm) and negligible at deeper locations (-1.1°/mm at 30 mm). The results from the tissue-mimicking phantom in scattering media indicate that angles between 30-75° could be used for imaging lesions at various depths (12 mm-28 mm) where lower LED illumination angles (closer to being parallel to the imaging plane) are preferable for deep tissue imaging and superficial lesion imaging is possible with higher LED illumination angles (closer to being perpendicular to the imaging plane). Our results can serve as a priori knowledge for the future LED-based PA system designs employed for both preclinical and clinical applications.

Dataset Information

Photoacoustic imaging of 3D-printed vascular networks.

Publications

Photoacoustic imaging of 3D-printed vascular networks.

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