Dataset Information

Genome-wide recombination map construction from single sperm sequencing in cattle.

ABSTRACT:

Background

Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle.

Results

To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle.

Conclusions

To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.

SUBMITTER: Yang L

PROVIDER: S-EPMC8898482 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Publications

Genome-wide recombination map construction from single sperm sequencing in cattle.

Yang Liu L Gao Yahui Y Li Mingxun M Park Ki-Eun KE Liu Shuli S Kang Xiaolong X Liu Mei M Oswalt Adam A Fang Lingzhao L Telugu Bhanu P BP Sattler Charles G CG Li Cong-Jun CJ Cole John B JB Seroussi Eyal E Xu Lingyang L Yang Lv L Zhou Yang Y Li Li L Zhang Hongping H Rosen Benjamin D BD Van Tassell Curtis P CP Ma Li L Liu George E GE

BMC genomics 20220305 1

<h4>Background</h4>Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle.<h4>Results</h4>To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of ...[more]

PMID: 35247961

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Project description:In eukaryotic cells, the production of cellular energy requires close interplay between nuclear and mitochondrial genomes. The mitochondrial genome is essential in that it encodes several genes involved in oxidative phosphorylation. Each cell contains several mitochondrial genome copies and mitochondrial DNA recombination is a widespread process occurring in plants, fungi, protists, and invertebrates. Saccharomyces cerevisiae has proved to be an excellent model to dissect mitochondrial biology. Several studies have focused on DNA recombination in this organelle, yet mostly relied on reporter genes or artificial systems. However, no complete mitochondrial recombination map has been released for any eukaryote so far. In the present work, we sequenced pools of diploids originating from a cross between two different S. cerevisiae strains to detect recombination events. This strategy allowed us to generate the first genome-wide map of recombination for yeast mitochondrial DNA. We demonstrated that recombination events are enriched in specific hotspots preferentially localized in non-protein-coding regions. Additionally, comparison of the recombination profiles of two different crosses showed that the genetic background affects hotspot localization and recombination rates. Finally, to gain insights into the mechanisms involved in mitochondrial recombination, we assessed the impact of individual depletion of four genes previously associated with this process. Deletion of NTG1 and MGT1 did not substantially influence the recombination landscape, alluding to the potential presence of additional regulatory factors. Our findings also revealed the loss of large mitochondrial DNA regions in the absence of MHR1, suggesting a pivotal role for Mhr1 in mitochondrial genome maintenance during mating. This study provides a comprehensive overview of mitochondrial DNA recombination in yeast and thus paves the way for future mechanistic studies of mitochondrial recombination and genome maintenance.

Dataset Information

Genome-wide recombination map construction from single sperm sequencing in cattle.

Background

Results

Conclusions

Publications

Genome-wide recombination map construction from single sperm sequencing in cattle.

Similar Datasets

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