Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
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ABSTRACT:
SUBMITTER: Stsiapanava A
PROVIDER: S-EPMC8930769 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Publications
Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
Stsiapanava Alena A Xu Chenrui C Nishio Shunsuke S Han Ling L Yamakawa Nao N Carroni Marta M Tunyasuvunakool Kathryn K Jumper John J de Sanctis Daniele D Wu Bin B Jovine Luca L
Nature structural & molecular biology 20220310 3
Glycoprotein 2 (GP2) and uromodulin (UMOD) filaments protect against gastrointestinal and urinary tract infections by acting as decoys for bacterial fimbrial lectin FimH. By combining AlphaFold2 predictions with X-ray crystallography and cryo-EM, we show that these proteins contain a bipartite decoy module whose new fold presents the high-mannose glycan recognized by FimH. The structure rationalizes UMOD mutations associated with kidney diseases and visualizes a key epitope implicated in cast ne ...[more]
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