Ontology highlight
ABSTRACT:
SUBMITTER: Laurent F
PROVIDER: S-EPMC89337 | biostudies-literature | 1999 Jul
REPOSITORIES: biostudies-literature
Laurent F F Poirel L L Naas T T Chaibi E B EB Labia R R Boiron P P Nordmann P P
Antimicrobial agents and chemotherapy 19990701 7
From genomic DNA of the clinical isolate Nocardia farcinica VIC, a 1. 6-kb Sau3AI fragment was cloned and expressed in Escherichia coli JM109. The recombinant strain expressed a beta-lactamase (pI, 4.6), FAR-1, which conferred high levels of resistance to amoxicillin, piperacillin, ticarcillin, and cephalothin. The hydrolysis constants (kcat, Km, Ki, and 50% inhibitory concentration) confirmed the MIC results and showed that FAR-1 activity is inhibited by clavulanic acid and at a low level by ta ...[more]