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Ultraviolet-B Irradiation Increases Antioxidant Capacity of Pakchoi (Brassica rapa L.) by Inducing Flavonoid Biosynthesis.

ABSTRACT: As an important abiotic stress factor, ultraviolet-B (UV-B) light can stimulate the accumulation of antioxidants in plants. In this study, the possibility of enhancing antioxidant capacity in pakchoi (Brassica rapa L.) by UV-B supplementation was assessed. Irradiation with 4 µmol·m-2·s-1 UV-B for 4 h or 2 µmol·m-2·s-1 UV-B for 24 h significantly increased the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and total reductive capacity, as a result of inducing a greater accumulation of total polyphenols and flavonoids without affecting the plant biomass. A high performance liquid chromatography (HPLC) analysis showed that the concentrations of many flavonoids significantly increased in response to UV-B treatment. The activities of three enzymes involved in the early steps of flavonoid biosynthesis, namely phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), and 4-coumarate: coenzyme A (CoA) ligase (4CL), were significantly increased after the corresponding UV-B treatment. Compared with the control, the expression levels of several flavonoid biosynthesis genes (namely BrPAL, BrC4H, Br4CL, BrCHS, BrF3H, BrF3'H, BrFLS, BrDFR, BrANS, and BrLDOX) were also significantly up-regulated in the UV-B treatment group. The results suggest that appropriate preharvest UV-B supplementation could improve the nutritional quality of greenhouse-grown pakchoi by promoting the accumulation of antioxidants.

SUBMITTER: Hao J

PROVIDER: S-EPMC8949486 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Ultraviolet-B Irradiation Increases Antioxidant Capacity of Pakchoi (Brassica rapa L.) by Inducing Flavonoid Biosynthesis.

Hao Juan J Lou Panpan P Han Yidie Y Zheng Lijun L Lu Jiangjie J Chen Zhehao Z Ni Jun J Yang Yanjun Y Xu Maojun M

Plants (Basel, Switzerland) 20220313 6

As an important abiotic stress factor, ultraviolet-B (UV-B) light can stimulate the accumulation of antioxidants in plants. In this study, the possibility of enhancing antioxidant capacity in pakchoi (Brassica rapa L.) by UV-B supplementation was assessed. Irradiation with 4 µmol·m-2·s-1 UV-B for 4 h or 2 µmol·m-2·s-1 UV-B for 24 h significantly increased the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and total reductive capacity, ...[more]

PMID: 35336648

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Dataset Information

Ultraviolet-B Irradiation Increases Antioxidant Capacity of Pakchoi (Brassica rapa L.) by Inducing Flavonoid Biosynthesis.

Publications

Ultraviolet-B Irradiation Increases Antioxidant Capacity of Pakchoi (<i>Brassica rapa</i> L.) by Inducing Flavonoid Biosynthesis.

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