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3D Printing of Monolithic Proteinaceous Cantilevers Using Regenerated Silk Fibroin.

ABSTRACT: Silk fibroin, regenerated from Bombyx mori, has shown considerable promise as a printable, aqueous-based ink using a bioinspired salt-bath system in our previous work. Here, we further developed and characterized silk fibroin inks that exhibit concentration-dependent fluorescence spectra at the molecular level. These insights supported extrusion-based 3D printing using concentrated silk fibroin solutions as printing inks. 3D monolithic proteinaceous structures with high aspect ratios were successfully printed using these approaches, including cantilevers only supported at one end. This work provides further insight and broadens the utility of 3D printing with silk fibroin inks for the microfabrication of proteinaceous structures.

SUBMITTER: Mu X

PROVIDER: S-EPMC9000323 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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3D Printing of Monolithic Proteinaceous Cantilevers Using Regenerated Silk Fibroin.

Mu Xuan X Gonzalez-Obeso Constancio C Xia Zhiyu Z Sahoo Jugal Kishore JK Li Gang G Cebe Peggy P Zhang Yu Shrike YS Kaplan David L DL

Molecules (Basel, Switzerland) 20220326 7

Silk fibroin, regenerated from Bombyx mori, has shown considerable promise as a printable, aqueous-based ink using a bioinspired salt-bath system in our previous work. Here, we further developed and characterized silk fibroin inks that exhibit concentration-dependent fluorescence spectra at the molecular level. These insights supported extrusion-based 3D printing using concentrated silk fibroin solutions as printing inks. 3D monolithic proteinaceous structures with high aspect ratios were ...[more]

PMID: 35408547

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Silk Fibroin Bioink for 3D Printing in Tissue Regeneration: Controlled Release of MSC extracellular Vesicles.

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