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Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest.

ABSTRACT: Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.

SUBMITTER: Uttarotai T

PROVIDER: S-EPMC9030188 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Alternative methods for restoring tropical forests influence the ecological processes that shape recruitment of understory species. In turn, the traits of species recruited will influence the ecological processes the forests provide now and over the long term. We assess the phylogenetic and functional structure of seedlings beneath monoculture plantations, mixed-species plantations (both active restoration) and regenerating selectively logged native forests (passive restoration), considering traits of specific leaf area (SLA, including within-species variation), leaf nitrogen and phosphorus content, life-form, potential plant height, and dispersal type. Monoculture plantations comprised seedlings that were more closely related then would be expected by chance (i.e., phylogenetically clustered), and regenerating forest contained species more distantly related then would be expected by chance (i.e., phylogenetically overdispersed). This suggests that seedlings beneath monocultures assemble through environmental filtering and through the dispersal limitation of predictable functional guilds. However, dispersal limitation is frequently overcome by human-assisted dispersal, increasing trait diversity. Comparing SLA values revealed that regenerating forests recruit seedlings with both high and low mean and variation of SLA, leading to higher overall diversity. Regenerating forest seedlings showed signs of environmental filtering, only based on within-species variation of SLA. Regenerating forest understories appear to favor species that show a high intraspecific variation in SLA values (e.g., Pterocarpus indicus Willd.) and at the same time provided habitat for later successional seedlings that show a lower intraspecific variation in SLA (e.g., Canarium luzonicum (Blume) A.Gray). This trait diversity suggests limiting similarity or competitive exclusion may be reduced because of niche differences, allowing species with different traits to coexist. Phylogenetic and functionally distinct species are restricted in their regeneration capacity, many of which are of conservation significance (under the IUCN Red List). Reforestation projects should maximize desired ecological services (including conservation value) by actively managing for the recruitment of species that are phylogenetically and functionally (including intraspecifically) distinct. This management aim will increase the probability of fulfilling a wider array of niche spaces and potentially increase the diversity of ecosystem services provided.

Dataset Information

Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest.

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