Dataset Information

Imaging of the skin microvascularization using spatially depolarized dynamic speckle.

ABSTRACT:

Significance

We propose a technique devoted to real-time high-resolution imaging of skin microvascularization.

Aim

The process utilizes the temporal variation of the spatially depolarized optical speckle field generated by moving red blood cells when illuminated with fully polarized coherent light.

Approach

Polarimetric filtering prevents the contribution of surface scattering from reaching the camera and thus favors the detection of multiscattered photons from the deeper layers of the skin.

Results

Full-field images reveal the microvasculature with a spatial resolution of 80 μm. The acquisition speed allows for real-time applications.

Conclusions

We demonstrate the ability of this method to determine in 1 s a stable and reliable microvascular activity, enabling numerous clinical applications that require quantitative measurements.

SUBMITTER: Colin E

PROVIDER: S-EPMC9043838 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Publications

Imaging of the skin microvascularization using spatially depolarized dynamic speckle.

Colin Elise E Plyer Aurélien A Golzio Muriel M Meyer Nicolas N Favre Gilles G Orlik Xavier X

Journal of biomedical optics 20220401 4

<h4>Significance</h4>We propose a technique devoted to real-time high-resolution imaging of skin microvascularization.<h4>Aim</h4>The process utilizes the temporal variation of the spatially depolarized optical speckle field generated by moving red blood cells when illuminated with fully polarized coherent light.<h4>Approach</h4>Polarimetric filtering prevents the contribution of surface scattering from reaching the camera and thus favors the detection of multiscattered photons from the deeper l ...[more]

PMID: 35478040

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Project description:PurposeTo assess the repeatability of blood flow velocity index (BFVi) metrics obtained with a recently Food and Drug Administration-cleared laser speckle contrast imaging device, the XyCAM RI (Vasoptic Medical, Inc), and to characterize differences in these metrics among control, glaucoma suspect, and glaucoma participants.DesignProspective, observational study.ParticipantsForty-six participants: 20 control, 16 glaucoma suspect, and 10 glaucoma participants, 1 eye per participant.MethodsKey dynamic BFVi metrics-mean, peak, dip, volumetric rise index (VRI), volumetric fall index (VFI), time to rise (TtR), time to fall (TtF), blow-out time (BOT), skew, and acceleration time index-were measured in the optic disc, optic disc vessels, optic disc perfusion region, and macula in 4 imaging sessions on the same day. Intrasession and intersession variability were calculated using the coefficient of variation (CV) for each metric in each region of interest (ROI). Values for each dynamic BFVi variable were compared between glaucoma, glaucoma suspect, and control participants using bivariate and multivariate analysis. Pearson correlation coefficients were used to correlate each variable in each ROI with age, intraocular pressure, cup-to-disc ratio (CDR), mean deviation, pattern standard deviation, retinal nerve fiber layer thickness, and minimum rim width.Main outcome measuresCoefficient of variation for the intrasession and intersession variability for each dynamic BFVi metric in each ROI and differences in each metric in each ROI between each diagnostic group.ResultsIntersession CV for mean, peak, dip, VRI, VFI, TtR, and TtF ranged from 3.2 ± 2.5% to 11.0 ± 3.8%. Age, CDR, OCT metrics, and visual field metrics showed significant correlations with dynamic BFVi variables. Peak, mean, dip, VRI, and VFI were significantly lower in patients with glaucoma than in control participants in all ROIs except the fovea. These metrics also were significantly lower in glaucoma patients than glaucoma suspect patients in the disc vessels.ConclusionsDynamic blood flow metrics measured with the XyCAM RI are reliable, are associated with structural and functional glaucoma metrics, and are significantly different among glaucoma, glaucoma suspect, and control participants. The XyCAM RI may serve as an important tool in glaucoma management in the future.

Dataset Information

Imaging of the skin microvascularization using spatially depolarized dynamic speckle.

Significance

Aim

Approach

Results

Conclusions

Publications

Imaging of the skin microvascularization using spatially depolarized dynamic speckle.

Similar Datasets

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