Dataset Information

Depletion of miR-96 Delays, But Does Not Arrest, Photoreceptor Development in Mice.

ABSTRACT:

Purpose

Abundant retinal microRNA-183 cluster (miR-183C) has been reported to be a key player in photoreceptor development and functionality in mice. However, whether there is a protagonist in this cluster remains unclear. Here, we used a mutant mouse model to study the role of miR-96, a member of miR-183C, in photoreceptor development and functionality.

Methods

The mature miR-96 sequence was removed using the CRISPR/Cas9 genome-editing system. Electroretinogram (ERG) and optical coherence tomography (OCT) investigated the changes in structure and function in mouse retinas. Immunostaining determined the localization and morphology of the retinal cells. RNA sequencing was conducted to observe retinal transcription alterations.

Results

The miR-96 mutant mice exhibited cone developmental delay, as occurs in miR-183/96 double knockout mice. Immunostaining of cone-specific marker genes revealed cone nucleus mislocalization and exiguous Opn1mw/Opn1sw in the mutant (MT) mouse outer segments at postnatal day 10. Interestingly, this phenomenon could be relieved in the adult stages. Transcriptome analysis revealed activation of microtubule-, actin filament-, and cilia-related pathways, further supporting the findings. Based on ERG and OCT results at different ages, the MT mice displayed developmental delay not only in cones but also in rods. In addition, a group of miR-96 potential direct and indirect target genes was summarized for interpretation and further studies of miR-96-related retinal developmental defects.

Conclusions

Depletion of miR-96 delayed but did not arrest photoreceptor development in mice. This miRNA is indispensable for mouse photoreceptor maturation, especially for cones.

SUBMITTER: Xiang L

PROVIDER: S-EPMC9055555 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Publications

Depletion of miR-96 Delays, But Does Not Arrest, Photoreceptor Development in Mice.

Xiang Lue L Zhang Juan J Rao Feng-Qin FQ Yang Qiao-Li QL Zeng Hui-Yi HY Huang Sheng-Hai SH Xie Zhen-Xiang ZX Lv Ji-Neng JN Lin Dan D Chen Xue-Jiao XJ Wu Kun-Chao KC Lu Fan F Huang Xiu-Feng XF Chen Qi Q

Investigative ophthalmology & visual science 20220401 4

<h4>Purpose</h4>Abundant retinal microRNA-183 cluster (miR-183C) has been reported to be a key player in photoreceptor development and functionality in mice. However, whether there is a protagonist in this cluster remains unclear. Here, we used a mutant mouse model to study the role of miR-96, a member of miR-183C, in photoreceptor development and functionality.<h4>Methods</h4>The mature miR-96 sequence was removed using the CRISPR/Cas9 genome-editing system. Electroretinogram (ERG) and optical ...[more]

PMID: 35481839

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Dataset Information

Depletion of miR-96 Delays, But Does Not Arrest, Photoreceptor Development in Mice.

Purpose

Methods

Results

Conclusions

Publications

Depletion of miR-96 Delays, But Does Not Arrest, Photoreceptor Development in Mice.

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