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Antioxidant activity of cerium dioxide nanoparticles and nanorods in scavenging hydroxyl radicals.

ABSTRACT: Cerium oxide nanoparticles (CeNPs) have been shown to exhibit antioxidant capabilities, but their efficiency in scavenging reactive oxygen species (ROS) and the underlying mechanisms are not yet well understood. In this study, cerium dioxide nanoparticles (CeNPs) and nanorods (CeNRs) were found to exhibit much stronger scavenging activity than ·OH generation in phosphate buffered saline (PBS) and surrogate lung fluid (SLF). The larger surface area and higher defect density of CeNRs may lead to higher ·OH scavenging activity than for CeNPs. These insights are important to understand the redox activity of cerium nanomaterials and provide clues to the role of CeNPs in biological and environmental processes.

SUBMITTER: Filippi A

PROVIDER: S-EPMC9063017 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Antioxidant activity of cerium dioxide nanoparticles and nanorods in scavenging hydroxyl radicals.

Filippi Alexander A Liu Fobang F Wilson Jake J Lelieveld Steven S Korschelt Karsten K Wang Ting T Wang Yueshe Y Reich Tobias T Pöschl Ulrich U Tremel Wolfgang W Tong Haijie H

RSC advances 20190409 20

Cerium oxide nanoparticles (CeNPs) have been shown to exhibit antioxidant capabilities, but their efficiency in scavenging reactive oxygen species (ROS) and the underlying mechanisms are not yet well understood. In this study, cerium dioxide nanoparticles (CeNPs) and nanorods (CeNRs) were found to exhibit much stronger scavenging activity than ·OH generation in phosphate buffered saline (PBS) and surrogate lung fluid (SLF). The larger surface area and higher defect density of CeNRs may lead to h ...[more]

PMID: 35520271

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Project description:Cerium dioxide (CeO2) and silicon dioxide (SiO2) nanoparticles are of widespread use in modern life. This means that human beings are markedly exposed to them in their everyday life. Once passing biological barriers, these nanoparticles are expected to interact with endothelial cells, leading to systemic alterations with distinct influences on human health. In the present study we observed the metabolic impact of differently sized CeO2 (8 nm; 35 nm) and SiO2 nanoparticles (117 nm; 315 nm) on immortalized human microvascular (HMEC-1) and primary macrovascular endothelial cells (HUVEC), with particular focus on the CeO2 nanoparticles. The characterization of the CeO2 nanoparticles in cell culture media with varying serum content indicated a steric stabilization of nanoparticles due to interaction with proteins. After cellular uptake, the CeO2 nanoparticles were localized around the nucleus in a ring-shaped manner. The nanoparticles revealed concentration and time, but no size-dependent effects on the cellular adenosine triphosphate levels. HUVEC reacted more sensitively to CeO2 nanoparticle exposure than HMEC-1. This effect was also observed in relation to cytokine release after nanoparticle treatment. The CeO2 nanoparticles exhibited a specific impact on the release of diverse proteins. Namely, a slight trend towards pro-inflammatory effects, a slight pro-thrombotic impact, and an increase of reactive oxygen species after nanoparticle exposure were observed with increasing incubation time. For SiO2 nanoparticles, concentration- and time-dependent effects on the metabolic activity as well as pro-inflammatory reactions were detectable. In general, the effects of the investigated nanoparticles on endothelial cells were rather insignificant, since the alterations on the metabolic cell activity became visible at a nanoparticle concentration that is by far higher than those expected to occur in the in vivo situation (CeO2 nanoparticles: 100 µg/mL; SiO2 nanoparticles: 10 µg/mL).

Project description:The antioxidant therapy to preserve residual hearing is relatively recent, and the search for effective antioxidants is still ongoing. Though nanoceria has shown promising radical-scavenging capability, improving its antioxidant ability and the dispersion stability of its nanofluid, which is critical to the desired site, i.e., cochlea, still remains a major challenge. The objective of the present work is to study the radical-scavenging capability of poly(ethylene glycol) (PEG)-coated CeO2 and Ce0.5Zr0.5O2 nanoparticles in water and the biologically relevant fluid (PBS buffer). Nanoparticles in the size range of 4.0-9.0 nm are synthesized using the coprecipitation method and characterized using suitable techniques. The scavenging and dispersion stability of the synthesized nanofluid are analyzed using a UV-vis spectrophotometer. It is found that the addition of PEG during the synthesis process promoted the generation of finer nanoparticles with a narrow size distribution and the doping of zirconium produced a large number of defects in the crystallite structure. The PEG coating over the nanoparticles improved the dispersion stability of nanofluids without affecting their surface reactivity, and it is found to be 94 and 80% in water and PBS, respectively, at 500 μM and 60 min, which is maintained till 90 min. The highest scavenging of hydroxyl radicals by PEG-coated Ce0.5Zr0.5O2 is found to be 60%, which is significantly superior to that of CeO2. The scavenging capability is found to be increased with the concentration of nanoparticles, showing the best scavenging activity at 190 and 150 μM for PEG-coated CeO2 and Ce0.5Zr0.5O2, respectively, and the scavenging in water is at par with that of PBS, indicating that these nanoparticles are suitable to be used in sites where a biologically relevant fluid is present, e.g., the cochlea. It is proposed that PEG-coated Ce0.5Zr0.5O2 having an average size of ∼ 4 nm can be a potential antioxidant in relevant biomedical applications.

Project description:In this study, cerium oxide nanorods (CeO2-NRs) were synthesized by using the phytochemicals present in the Dalbergia sissoo extract. The physiochemical characteristics of the as-prepared CeO2-NRs were investigated by using ultraviolet-visible spectroscopy (UV-VIS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD). The SEM and UV-VIS analyses revealed that the acquired nanomaterials possessed a rod-like morphology while the XRD results further confirmed that the synthesized NRs exhibited a cubic crystal lattice system. The antioxidant capacity of the synthesized CeO2-NRs was investigated by using several in vitro biochemical assays. It was observed that the synthesized NRs exhibited better antioxidant potential in comparison to the industrial antioxidant of the butylated hydroxyanisole (BHA) in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The biochemical assays, including lipid peroxidation (LPO), total antioxidant capacity (TAC), and catalase activity (CAT), were also performed in the human lymphocytes incubated with the CeO2-NRs to investigate the impact of the NRs on these oxidative biomarkers. Enhanced reductive capabilities were observed in all the assays, revealing that the NRs possess excellent antioxidant properties. Moreover, the cytotoxic potential of the CeO2-NRs was also investigated with the MTT assay. The CeO2-NRs were found to effectively kill off the cancerous cells (MCF-7 human breast cancer cell line), further indicating that the synthesized NRs exhibit anticancer potential as well. One of the major applications studied for the prepared CeO2-NRs was performing the statistical optimization of the photocatalytic degradation reaction of the methyl orange (MO) dye. The reaction was optimized by using the technique of response surface methodology (RSM). This advanced approach facilitates the development of the predictive model on the basis of central composite design (CCD) for this degradation reaction. The maximum degradation of 99.31% was achieved at the experimental optimized conditions, which corresponded rather well with the predicted percentage degradation values of 99.58%. These results indicate that the developed predictive model can effectively explain the performed experimental reaction. To conclude, the CeO2-NRs exhibited excellent results for multiple applications.

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Antioxidant activity of cerium dioxide nanoparticles and nanorods in scavenging hydroxyl radicals.

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Antioxidant activity of cerium dioxide nanoparticles and nanorods in scavenging hydroxyl radicals.

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