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Surface analysis of curved polymeric plates irradiated with proton and ion beams.

ABSTRACT: We explored surface characteristics of polycarbonate substrates which were subjected to proton and nitrogen ion beams. Curved plates designed for a plastic window of a cell phone were fabricated using injection molding, and the process was simulated numerically. Different energy densities of proton and nitrogen ion beams were applied in this study. The physical and chemical properties of the samples were evaluated using UV-Vis spectroscopy, FT-IR, differential scanning calorimetry (DSC), nanoindentation, residual stress, and electrical analyses. Results revealed that the irradiation of proton and nitrogen ion beams led to changes in the chemical structures and energy levels of the polymer molecules. Furthermore, physical properties, such as hardness, glass transition temperature, transmittance, and electrical conductivity, were remarkably varied.

SUBMITTER: Song YS

PROVIDER: S-EPMC9087293 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Surface analysis of curved polymeric plates irradiated with proton and ion beams.

Song Young Seok YS Kang Chul C Jeong Jiwon J Kim Minji M Lim Eunju E

RSC advances 20181011 61

We explored surface characteristics of polycarbonate substrates which were subjected to proton and nitrogen ion beams. Curved plates designed for a plastic window of a cell phone were fabricated using injection molding, and the process was simulated numerically. Different energy densities of proton and nitrogen ion beams were applied in this study. The physical and chemical properties of the samples were evaluated using UV-Vis spectroscopy, FT-IR, differential scanning calorimetry (DSC), nanoind ...[more]

PMID: 35547031

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Project description:Proton therapy allows to avoid excess radiation dose on normal tissues. However, there are some limitations. Indeed, passive delivery of proton beams results in an increase in the lateral dose upstream of the tumor and active scanning leads to strong differences in dose delivery. This study aims to assess possible differences in the transcriptomic response of skin in C57BL/6 mice after TBI irradiation by active or passive proton beams at the dose of 6 Gy compared to unirradiated mice. In that purpose, total RNA was extracted from skin samples 3 months after irradiation and RNA-Seq was performed. Results showed that active and passive delivery lead to completely different transcription profiles. Indeed, 140 and 167 genes were differentially expressed after active and passive scanning compared to unirradiated, respectively, with only one common gene corresponding to RIKEN cDNA 9930021J03. Moreover, protein-protein interactions performed by STRING analysis showed that 31 and 25 genes are functionally related after active and passive delivery, respectively, with no common gene between both types of proton delivery. Analysis showed that active scanning led to the regulation of genes involved in skin development which was not the case with passive delivery. Moreover, 14 ncRNA were differentially regulated after active scanning against none for passive delivery. Active scanning led to 49 potential mRNA-ncRNA pairs with one ncRNA mainly involved, Gm44383 which is a miRNA. The 43 genes potentially regulated by the miRNA Gm44393 confirmed an important role of active scanning on skin keratin pathway. Our results demonstrated that there are differences in skin gene expression still 3 months after proton irradiation versus unirradiated mouse skin. And strong differences do exist in late skin gene expression between scattered or scanned proton beams. Further investigations are strongly needed to understand this discrepancy and to improve treatments by proton therapy.

Project description:ObjectiveHeavy ions have contributed to tumor site-specific radiotherapy and are a major health risk for astronauts. The purpose of this study was to investigate the changes in gene expression in peripheral lymphocytes of cancer patients and astronauts exposed to 12C ions, and identify suitable molecular biomarkers for health monitoring. We also aimed to observe the effects of treatment and the level of damage, by comparing the transcriptional profiles of human lymphocyte cell lines exposed to 12C ion beams at doses of 0-2.0 Gy.Materials and methodsA human lymphocyte cell line was irradiated with 12C ion beams at 0, 0.1, 0.5, and 2.0 Gy and transcriptional profiles were evaluated using the Agilent human gene expression microarray at 24 hours after irradiation. Differentially expressed genes were identified using a fold change of ≥2.0. Representative genes were further validated by RT-PCR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to determine the roles of differentially expressed mRNAs.ResultsBased on the microarray assays, 1,113 genes were upregulated and 853 genes were downregulated in human lymphocyte cells irradiated with 0.1 Gy 12C ion beams compared with the control group, 1,095 genes were upregulated and 1,220 genes were downregulated in cells irradiated with 0.5 Gy 12C ion beams, and 1,055 genes were upregulated and 1,356 genes were downregulated in cells irradiated with 2.0 Gy. A total of 504 genes were differentially expressed in all irradiated groups, of which 88 genes were upregulated and 416 genes downregulated. Most of these altered genes were related to the cell cycle, apoptosis, signal transduction, DNA transcription, repair, and replication. The expression differences were further confirmed by RT-PCR for a subset of differentially expressed genes.ConclusionDifferentially expressed genes between treatment and control groups at 24 hours post-irradiation increased as the radiation dose increased; upregulated genes gradually decreased and downregulated genes increased. Our data indicated that 12C ion beams could repress a number of genes in a dose-dependent manner, which might lead to the failure of multiple cellular biological functions.

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Surface analysis of curved polymeric plates irradiated with proton and ion beams.

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Surface analysis of curved polymeric plates irradiated with proton and ion beams.

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