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Generation and characterization of humanized synergistic neutralizing antibodies against SARS-CoV-2.

ABSTRACT: The emerging coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the causative agent of coronavirus disease 2019 (COVID-19), which has become a severe threat to global public health and local economies. In this study, several single-chain antibody fragments that bind to the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein were identified and used to construct human-mouse chimeric antibodies and humanized antibodies. These antibodies exhibited strong binding to RBD and neutralization activity towards a SARS-CoV-2 pseudovirus. Moreover, these antibodies recognize different RBD epitopes and exhibit synergistic neutralizing activity. These provide candidate to combination use or bispecific antibody to potential clinical therapy for COVID-19.

SUBMITTER: Guo J

PROVIDER: S-EPMC9088636 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Generation and characterization of humanized synergistic neutralizing antibodies against SARS-CoV-2.

Guo Jiazheng J Zhang Jun J Du Peng P Lu Jiansheng J Chen Lei L Huang Ying Y Yu Yunzhou Y Xie Qing Q Wang Rong R Yang Zhixin Z

Journal of medical virology 20220507 8

The emerging coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the causative agent of coronavirus disease 2019 (COVID-19), which has become a severe threat to global public health and local economies. In this study, several single-chain antibody fragments that bind to the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein were identified and used to construct human-mouse chimeric antibodies and humanized antibodies. These antibodies exhibited strong bin ...[more]

PMID: 35451094

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