Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.
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ABSTRACT:
SUBMITTER: Kim SH
PROVIDER: S-EPMC91015 | biostudies-literature | 1999 Jan
REPOSITORIES: biostudies-literature
Publications
Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.
Kim S H SH Uzunovic A A Breuil C C
Applied and environmental microbiology 19990101 1
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi. ...[more]
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