In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture.
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ABSTRACT:
SUBMITTER: Pathak RU
PROVIDER: S-EPMC9117923 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
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<i>In situ</i> Nuclear Matrix preparation in <i>Drosophila melanogaster</i> enabling genetic analysis of the nuclear architecture.
Pathak Rashmi U RU Bihani Ashish A Sureka Rahul R Mishra Rakesh K RK
STAR protocols 20220513 2
Nuclear Matrix (NuMat) is a biochemically defined entity that provides us with a snapshot of the features of the nuclear architecture. Here, we present a protocol to isolate and visualize NuMat <i>in situ</i> in the intact embryo or tissues of <i>Drosophila melanogaster</i> and its applications. We remove the chromatin to reveal underlying nuclear architectural components in organismal context. This protocol couples the power of <i>Drosophila</i> genetics with cell biological observation of the ...[more]
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