Dataset Information

Drosophila melanogaster nuclear matrix proteome

ABSTRACT: Nucleus is a highly structured organelle and contains many functional compartments. While the structural basis for this complex spatial organization of compartments is unknown, a major component of this organization is likely to be the non-chromatin scaffolding called nuclear matrix (NuMat). Experimental evidence over the past decades indicates that most of the nuclear functions are at least transiently associated with the NuMat although the components of NuMat itself are poorly known. Here, we report NuMat proteome analysis from Drosophila melanogaster embryos and discuss its links with nuclear architecture and functions. In the NuMat proteome, we find structural proteins, chaperones related, DNA/RNA binding, chromatin remodeling and transcription factors. This complexity of NuMat proteome is an indicator of its structural and functional significance. Comparison of the 2D profile of NuMat proteome from different developmental stages of Drosophila embryos shows that less than half of the NuMat proteome is constant and rest of the proteins are stage specific dynamic components. This NuMat dynamics suggests a possible functional link between NuMat and the embryonic development. Finally, we also show that a subset of NuMat proteins remain associated with the mitotic chromosomes implicating their role in mitosis and possibly the epigenetic cellular memory. NuMat proteome analysis provides tools and opens up ways to understand nuclear organization and function.

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Rakesh Mishra

PROVIDER: PRD000255 | Pride | 2011-05-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_12879.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_12879.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_12880.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_12880.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_12881.pride.mgf.gz	Mgf

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Publications

Nuclear matrix proteome analysis of Drosophila melanogaster.

Kallappagoudar Satish S Varma Parul P Pathak Rashmi Upadhyay RU Senthilkumar Ramamoorthy R Mishra Rakesh K RK

Molecular & cellular proteomics : MCP 20100608 9

The nucleus is a highly structured organelle and contains many functional compartments. Although the structural basis for this complex spatial organization of compartments is unknown, a major component of this organization is likely to be the non-chromatin scaffolding called nuclear matrix (NuMat). Experimental evidence over the past decades indicates that most of the nuclear functions are at least transiently associated with the NuMat, although the components of NuMat itself are poorly known. H ...[more]

PMID: 20530634

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