Dataset Information

Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease.

ABSTRACT:

Background and aims

Subjects with congenital chloride diarrhea (CLD; a defect in solute carrier family 26 member 3 (SLC26A3)) are prone to inflammatory bowel disease (IBD). We investigated fecal microbiota in CLD and CLD-associated IBD. We also tested whether microbiota is modulated by supplementation with the short-chain fatty acid butyrate.

Subjects and methods

We recruited 30 patients with CLD for an observational 3-week follow-up study. Thereafter, 16 consented to oral butyrate substitution for a 3-week observational period. Fecal samples, collected once a week, were assayed for calprotectin and potential markers of inflammation, and studied by 16S ribosomal ribonucleic acid (rRNA) gene amplicon sequencing and compared to that of 19 healthy controls and 43 controls with Crohn's disease. Data on intestinal symptoms, diet and quality of life were collected.

Results

Patients with CLD had increased abundances of Proteobacteria, Veillonella, and Prevotella, and lower abundances of normally dominant taxa Ruminococcaceae and Lachnospiraceae when compared with healthy controls and Crohn´s disease. No major differences in fecal microbiota were found between CLD and CLD-associated IBD (including two with yet untreated IBD). Butyrate was poorly tolerated and showed no major effects on fecal microbiota or biomarkers in CLD.

Conclusions

Fecal microbiota in CLD is different from that of healthy subjects or Crohn´s disease. Unexpectedly, no changes in the microbiota or fecal markers characterized CLD-associated IBD, an entity with high frequency among patients with CLD.

SUBMITTER: Wedenoja S

PROVIDER: S-EPMC9182261 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease.

Wedenoja Satu S Saarikivi Aki A Mälkönen Jani J Leskinen Saara S Lehto Markku M Adeshara Krishna K Tuokkola Jetta J Nikkonen Anne A Merras-Salmio Laura L Höyhtyä Miikka M Hörkkö Sohvi S Haaramo Anu A Salonen Anne A de Vos Willem M WM Korpela Katri K Kolho Kaija-Leena KL

PloS one 20220609 6

<h4>Background and aims</h4>Subjects with congenital chloride diarrhea (CLD; a defect in solute carrier family 26 member 3 (SLC26A3)) are prone to inflammatory bowel disease (IBD). We investigated fecal microbiota in CLD and CLD-associated IBD. We also tested whether microbiota is modulated by supplementation with the short-chain fatty acid butyrate.<h4>Subjects and methods</h4>We recruited 30 patients with CLD for an observational 3-week follow-up study. Thereafter, 16 consented to oral butyrat ...[more]

PMID: 35679312

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Dataset Information

Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease.

Background and aims

Subjects and methods

Results

Conclusions

Publications

Fecal microbiota in congenital chloride diarrhea and inflammatory bowel disease.

Similar Datasets

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