Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region.
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ABSTRACT:
SUBMITTER: Dobner P
PROVIDER: S-EPMC228907 | biostudies-other | 1996 Apr
REPOSITORIES: biostudies-other
Publications
Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region.
Dobner P P Feldmann K K Rifai M M Löscher T T Rinder H H
Journal of clinical microbiology 19960401 4
A total of 0.3 to 0.4 kb of the promoter region of the 16S rRNA genes from Mycobacterium tuberculosis, M. gordonae, M. xenopi, and M. leprae was PCR amplified, cloned, and sequenced. The observed number of substitutions, insertions, and deletions exceeded those found in previously used target sequences, including the entire 16S coding region. A simple and generally applicable restriction fragment length polymorphism method that can be used to distinguish between mycobacterial species is describe ...[more]
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Project description:Development of an analysis pipeline characterizing multiple hypervariable regions of 16S rRNA using mock samples
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