Spinach-based RNA mimicking GFP in plant cells.
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ABSTRACT:
SUBMITTER: Yu Z
PROVIDER: S-EPMC9197860 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Spinach-based RNA mimicking GFP in plant cells.
Yu Zhiming Z Wang Yue Y Mei Fengling F Yan Haiting H Jin Zhenhui Z Zhang Pengcheng P Zhang Xian X Tör Mahmut M Jackson Stephen S Shi Nongnong N Hong Yiguo Y
Functional & integrative genomics 20220310 3
Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNA<sup>Lys</sup> can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNA<sup>Lys</sup>-Spinach-t ...[more]
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