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Spinach RNA aptamer detects lead(II) with high selectivity.

ABSTRACT: Spinach RNA aptamer contains a G-quadruplex motif that serves as a platform for binding and fluorescence activation of a GFP-like fluorophore. Here we show that Pb(2+) induces formation of Spinach's G-quadruplex and activates fluorescence with high selectivity and sensitivity. This device establishes the first example of an RNA-based sensor that provides a simple and inexpensive tool for Pb(2+) detection.

SUBMITTER: DasGupta S

PROVIDER: S-EPMC4755583 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Spinach RNA aptamer detects lead(II) with high selectivity.

DasGupta Saurja S Shelke Sandip A SA Li Nan-sheng NS Piccirilli Joseph A JA

Chemical communications (Cambridge, England) 20150501 43

Spinach RNA aptamer contains a G-quadruplex motif that serves as a platform for binding and fluorescence activation of a GFP-like fluorophore. Here we show that Pb(2+) induces formation of Spinach's G-quadruplex and activates fluorescence with high selectivity and sensitivity. This device establishes the first example of an RNA-based sensor that provides a simple and inexpensive tool for Pb(2+) detection. ...[more]

PMID: 25940073

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