Microarray analysis of long non-coding RNAs related to osteogenic differentiation of human dental pulp stem cells.

ABSTRACT:

Background/purpose

Dental pulp stem cells (DPSCs) are candidate seed cells for bone tissue engineering, but the molecular regulation of osteogenic differentiation in DPSCs is not fully understood. Long non-coding RNAs (lncRNAs) are important regulators of gene expression, and whether they play roles in osteogenic differentiation of DPSCs requires more study.

Materials and methods

DPSCs were isolated and cultured. The mRNA and lncRNA expression profiles were compared through microarray assay between osteo-differentiated DPSCs and non-differentiated DPSCs. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, Gene ontology (GO) analyses, and the mRNA-lncRNA co-expression analyses were performed for functional annotation of differentially expressed RNAs. Small interfering RN

SUBMITTER: Hao X 

PROVIDER: S-EPMC9201533 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature