Synthesis of encapsulated ZnO nanowires provide low impedance alternatives for microelectrodes.
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ABSTRACT:
SUBMITTER: Maddah M
PROVIDER: S-EPMC9202946 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:ObjectiveRecording electrical activity from individual cells in vivo is a key technology for basic neuroscience and has growing clinical applications. To maximize the number of independent recording channels as well as the longevity, and quality of these recordings, researchers often turn to small and flexible electrodes that minimize tissue damage and can isolate signals from individual neurons. One challenge when creating these small electrodes, however, is to maintain a low interfacial impedance by applying a surface coating that is stable in tissue and does not significantly complicate the fabrication process.ApproachHere we use a high-pressure Pt sputtering process to create low-impedance electrodes at the wafer scale using standard microfabrication equipment.Main resultsWe find that direct-sputtered Pt provides a reliable and well-controlled porous coating that reduces the electrode impedance by 5-9 fold compared to flat Pt and is compatible with the microfabrication technologies used to create flexible electrodes. These porous Pt electrodes show reduced thermal noise that matches theoretical predictions. In addition, we show that these electrodes can be implanted into rat cortex, record single unit activity, and be removed all without disrupting the integrity of the coating. We also demonstrate that the shape of the electrode (in addition to the surface area) has a significant effect on the electrode impedance when the feature sizes are on the order of tens of microns.SignificanceOverall, porous Pt represents a promising method for manufacturing low-impedance electrodes that can be seamlessly integrated into existing processes for producing flexible neural probes.
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Project description:Transparent microelectrodes that facilitate simultaneous optical and electrophysiological interfacing are desirable tools for neuroscience. Electrodes made from transparent conductors such as graphene and indium tin oxide (ITO) show promise but are often limited by poor interfacial charge-transfer properties. Here, microelectrodes are demonstrated that take advantage of the transparency and volumetric capacitance of the mixed ion-electron conductor Poly(3,4- ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS). Ring-shaped microelectrodes are fabricated by inkjet-printing PEDOT:PSS, encapsulating with Parylene-C, and then exposing a contact site that is much smaller than the microelectrode outer diameter. This unique structure allows the encapsulated portion of the microelectrode volume surrounding the contact site to participate in signal transduction, which reduces impedance and enhances charge storage capacity. While using the same 100 μm diameter contact site, increasing the outer diameter of the encapsulated electrode from 300 to 550 μm reduces the impedance from 294±21 to 98±2 kΩ, respectively, at 1 Hz. Similarly, the charge storage capacity is enhanced from 6 to 21 mC cm−2. The PEDOT:PSS microelectrodes provide a low-haze, high-transmittance optical interface, demonstrating their suitability for optical neuroscience applications. They remain functional after a million 1 V stimulation cycles, up to 600 μA of stimulation current, and more than 1000 mechanical bending cycles. ToC Entry: Inkjet-printed transparent microelectrodes composed of a mixed-conductor polyelectrolyte are demonstrated and characterized according to their electrochemical, optical, and mechanical properties. It is shown that the impedance and charge storage capacity of these microelectrodes can be improved by increasing the volume of the microelectrode without altering the contact site area or the transparency.
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