Ontology highlight
ABSTRACT:
SUBMITTER: Borchert AJ
PROVIDER: S-EPMC9208016 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Borchert Andrew J AJ Bleem Alissa A Beckham Gregg T GT
ACS synthetic biology 20220603 6
Randomly barcoded transposon insertion sequencing (RB-TnSeq) is an efficient, multiplexed method to determine microbial gene function during growth under a selection condition of interest. This technique applies to growth, tolerance, and persistence studies in a variety of hosts, but the wealth of data generated can complicate the identification of the most critical gene targets. Experimental and analytical methods for improving the resolution of RB-TnSeq are proposed, using <i>Pseudomonas putid ...[more]