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Isolation and characterization of 2,3-dichloro-1-propanol-degrading rhizobia.

ABSTRACT: 2,3-Dichloro-1-propanol is more chemically stable than its isomer, 1, 3-dichloro-2-propanol, and is therefore more difficult to degrade. The isolation of bacteria capable of complete mineralization of 2, 3-dichloro-1-propanol was successful only from enrichments at high pH. The bacteria thus isolated were found to be members of the alpha division of the Proteobacteria in the Rhizobium subdivision, most likely Agrobacterium sp. They could utilize both dihaloalcohol substrates and 2-chloropropionic acid. The growth of these strains in the presence of 2,3-dichloro-1-propanol was strongly affected by the pH and buffer strength of the medium. Under certain conditions, a ladder of four active dehalogenase bands could be visualized from this strain in activity gels. The enzyme involved in the complete mineralization of 2,3-dichloro-1-propanol was shown to have a native molecular weight of 114,000 and consisted of four subunits of similar molecular weights.

SUBMITTER: Effendi AJ

PROVIDER: S-EPMC92087 | biostudies-literature | 2000 Jul

REPOSITORIES: biostudies-literature

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Isolation and characterization of 2,3-dichloro-1-propanol-degrading rhizobia.

Effendi A J AJ Greenaway S D SD Dancer B N BN

Applied and environmental microbiology 20000701 7

2,3-Dichloro-1-propanol is more chemically stable than its isomer, 1, 3-dichloro-2-propanol, and is therefore more difficult to degrade. The isolation of bacteria capable of complete mineralization of 2, 3-dichloro-1-propanol was successful only from enrichments at high pH. The bacteria thus isolated were found to be members of the alpha division of the Proteobacteria in the Rhizobium subdivision, most likely Agrobacterium sp. They could utilize both dihaloalcohol substrates and 2-chloropropioni ...[more]

PMID: 10877782

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Novel dehalogenase mechanism for 2,3-dichloro-1-propanol utilization in Pseudomonas putida strain MC4.

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Isolation and characterization of rhizobia from nodules of Clitoria ternatea in Thailand.

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Isolation and characterization of phenol-degrading denitrifying bacteria.

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Isolation and Characterization of Phenol-Degrading Psychrotolerant Yeasts.

Project description:In this study, the potential of selected psychrotolerant yeast strains for phenol biodegradation was studied. From 39 strains isolated from soil and water samples from Rucianka peat bog, three psychrotolerant yeast strains, A011, B021, and L012, showed the ability to degrade phenol. The result shows that all three yeast strains could degrade phenol at 500 and 750 mg l-1 concentration, whereas strains A011 and L012 could degrade phenol at 1000 mg l-1 concentration. The time needed for degradation of each phenol concentration was no longer than 2 days. Strains A011, B021, and L012 were identified based on 26S rDNA and ITS sequence analysis as belonging to species Candida subhashii, Candida oregonensis, and Schizoblastosporion starkeyi-henricii, respectively.

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2,4-Dichloro-N-(2,3-dichloro-phen-yl)benzene-sulfonamide.

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2,3-Dichloro-3',4'-dihydroxybiphenyl.

Project description:2,3-Dichloro-3',4'-dihydroxybiphenyl, C12H8Cl2O2, is a putative dihydroxylated metabolite of 2,3-dichlorobiphenyl (PCB 5). The title structure displays intramolecular O-H···O hydrogen bonding, and the ?-? stacking distance between inversion related chlorinated benzene rings of the title compound is 3.371 (3) Å. The dihedral angle between two benzene rings is 59.39 (8)°.

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1,4-Dichloro-naphthalene-2,3-diol.

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2,3-Dichloro-benzene-1,4-diol.

Project description:The achiral title compound, C(6)H(4)Cl(2)O(2), crystallizes with O-H?O hydrogen bonding linking mol-ecules into layers. Between layers there are chains of Cl?Cl?Cl inter-actions with alternating distances of 3.274?(2) and 3.742?(2)?Å. Augmenting this arrangement there are also C-H?Cl (2.97 and 3.17?Å) and Cl?? (shortest distances 3.40 and 3.54?Å) inter-actions.

| S-EPMC2977781 | biostudies-literature

Isolation, characterization and application of theophylline-degrading Aspergillus fungi.

Project description:BACKGROUND:Caffeine, theobromine and theophylline are main purine alkaloid in tea. Theophylline is the downstream metabolite and it remains at a very low level in Camellia sinensis. In our previous study, Aspergillus sydowii could convert caffeine into theophylline in solid-state fermentation of pu-erh tea through N-demethylation. In this study, tea-derived fungi caused theophylline degradation in the solid-state fermentation. The purpose of this study is identify and isolate theophylline-degrading fungi and investigate their application in production of methylxanthines with theophylline as feedstock through microbial conversion. RESULTS:Seven tea-derived fungi were collected and identified by ITS, ?-tubulin and calmodulin gene sequences, Aspergillus ustus, Aspergillus tamarii, Aspergillus niger and A. sydowii associated with solid-state fermentation of pu-erh tea have shown ability to degrade theophylline in liquid culture. Particularly, A. ustus and A. tamarii could degrade theophylline highly significantly (p?<?0.01). 1,3-dimethyluric acid, 3-methylxanthine, 3-methyluric acid, xanthine and uric acid were detected consecutively by HPLC in A. ustus and A. tamarii, respectively. The data from absolute quantification analysis suggested that 3-methylxanthine and xanthine were the main degraded metabolites in A. ustus and A. tamarii, respectively. 129.48?±?5.81 mg/L of 3-methylxanthine and 159.11?±?10.8 mg/L of xanthine were produced by A. ustus and A. tamarii in 300 mg/L of theophylline liquid medium, respectively. CONCLUSIONS:For the first time, we confirmed that isolated A. ustus, A. tamarii degrade theophylline through N-demethylation and oxidation. We were able to biologically produce 3-methylxanthine and xanthine efficiently from theophylline through a new microbial synthesis platform with A. ustus and A. tamarii as appropriate starter strains.

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