Quantitative RNA assessment and long-term stability in the FFPE tumor samples using Digital Spatial Profiler.

ABSTRACT:

Background

Long-term storage of tissue slides has been reported to induce reduced biomarker (e.g. proteins and messenger RNA) detection. This study aimed to evaluate the impact of long-term storage time (0, 16, 24 and 36 weeks) and treatment conditions (non-paraffin and paraffin dipping) at 4°C on RNA quality in formalin-fixed and paraffin-embedded (FFPE) tissue sections.

Materials and methods

NanoString GeoMx Digital Spatial Profiling (DSP), a novel platform that allows spatial profiling, was used to profile RNA in human bladder cancer FFPE tissue sections.

Results

We observed excellent consistency of quantitative DSP RNA counts of all targets between two different treatment conditions (R > 0.97, Pearson correlation) at each time point and among all four diffe

SUBMITTER: Gupta S 

PROVIDER: S-EPMC9216648 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature