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Molecular phylogeny of Anabaena circinalis and its identification in environmental samples by PCR.

ABSTRACT: Although the cyanobacterium Anabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification of A. circinalis in a mixed population is complicated due to limited morphological differences between Anabaena species. Sequence analysis of the DNA-dependent RNA polymerase (rpoC1) gene from 24 Anabaena isolates, including 12 designated A. circinalis, permitted a phylogenetic analysis to be performed. In addition, an A. circinalis-specific PCR was developed and tested successfully on environmental samples.

SUBMITTER: Fergusson KM

PROVIDER: S-EPMC92275 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature

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Molecular phylogeny of Anabaena circinalis and its identification in environmental samples by PCR.

Fergusson K M KM Saint C P CP

Applied and environmental microbiology 20000901 9

Although the cyanobacterium Anabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification of A. circinalis in a mixed population is complicated due to limited morphological differences between Anabaena species. Sequence analysis of the DNA-dependent RNA polymerase (rpoC1) gene from 24 Anabaena isolates, including 12 designated A. circinalis, permitted a phylogenetic analysis to be perfor ...[more]

PMID: 10966445

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