Modeling myosin Va liposome transport through actin filament networks reveals a percolation threshold that modulates transport properties.

ABSTRACT: Myosin Va (myoVa) motors transport membrane-bound cargo through three-dimensional, intracellular actin filament networks. We developed a coarse-grained, in silico model to predict how actin filament density (3-800 filaments) within a randomly oriented actin network affects fluid-like liposome (350 nm vs. 1750 nm) transport by myoVa motors. Five thousand simulated liposomes transported within each network adopted one of three states: transport, tug-of-war, or diffusion. Diffusion due to liposome detachment from actin rarely occurred given at least 10 motors on the liposome surface. However, with increased actin density, liposomes transitioned from primarily directed transport on single actin filaments to an apparent random walk, resulting from a mixture of transport and tug-of-wars as the p

SUBMITTER: Walcott S 

PROVIDER: S-EPMC9236151 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature